If I add 10 microliters of a culture at 106 cells/mL to 990 microliters of nutrientbroth, what is the cell density in the new solution?

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Q: to dilute a sample by 1/3. For a final volume of 27 ml, how much sample would you add to how much…

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Q: What is the appropriate dose (chlorine, ozone, ultraviolet rays) to sterilize water per 100 cubic…

A: Hi! Thank you for the question! Since the appropriate dosage of ultraviolet rays is not known, the…

Q: What is pH and EC of nutrient solution and why they need to be measured?

A: Answer: Introduction: pH is a negative logarithm of hydrogen ion concentration in each solution.

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: If the pH indicator were left out of MacConkey agar, the medium would bea) complex.b)…

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: How is the COD Values be affected in the presence of the following contaminants in your sample?…

A: COD value means chemical oxygen demand. The COD value indicates  the amount of oxygen which is…

Q: What is the bulk density of a 100 cubic centimeter soil sample that weighs 150 grams?

A: Bulk density is otherwise known as Volumetric density. It is defined as the weight of soil in…

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A: Yes ammonia nitrogen level increase in waste water which was prior stored without acidification.

Q: During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why…

A: For experiments involving growth of cells whether bacterial or eukaryotic the results of a growth…

Q: Explain why high concentration of suger, salt and acid do not support microbial growth, only it have…

A: Microbial Growth refers to the growth of a microbial population i.e., an increase in its cell number…

Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?

A: Utilising the membrane filter method, test is gone through the layer utilising a filter funnel and…

Q: What volume of water is needed to make 40uL of 100ug/mL BSA from a 2mg/mL BSA stock? Group of answer…

A: C1V1=C2V2 is used to calculate an unknown quantity where two solutions/mixtures are proportional

Q: You have a 15% (m/v) solution put any units in your answer, ju

A: Dilution Equation M1 V1 = M2  V2

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A: Fatty acid methyl esters or FAME are a type of fatty acid esters. This is formed by the…

Q: During the recovery stage of annealing, the grain size Select] v and dislocations [ Select)…

A: 1  Increases 2 increase in density

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A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: Why should Erlenmeyer flasks hold not more than 60% of their capacity?  When can gelatin be used in…

A: 1*Erlenmeyer flasks are used to contain liquids and for mixing and heating and cooling, incubation…

Q: If you added 5 mL of a 20X tocopherol to 5 mL of cell culture at a density of 2.4 x 107 cells/mL,…

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Q: solve for the cfu/ml in a given of 300 colonies at plate 10^4. show complete solution. see attached…

A: CFU or colony forming unit is the viable count of bacterial/fungal cells. In direct microscopic…

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Q: if you are going to prepare 15 plates of Nutrient Agar, 30 NA slants, and 20 NA stabs, how much tap…

A: In usual labs petriplatea can hold 15ml of media therefore for 15plates approximately 15×15 = 225ml…

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Q: Why do the leaf disks in the baking soda solution (treatment) begin to float?

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Q: Using the following absorbance data set, would you c Salt concentration Organism A Organism B 1%…

A: YES.

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A: The tiny organisms that cannot be observed by unaided eyes are the microorganisms. The microbes…

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

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Q: 7.3x105 CFU/ml= Broth culture, how many bacteria would be contained

A: The number of viable bacterial cells in liquid culture is estimated by means of a colony-forming…

Q: What is the substrate(s) that will be used by the microorganism to produce the calcite ?

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A: Introduction The colony forming unit (CFU) is a measurement indicating the number of viable…

Q: If I add 10 microliters of a culture at 106 cells/mL to 990 microliters of nutrient broth, what is…

A: Cell density refers to number of cells present per unit volume. It is usually expressed in cells/mL.

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A: The shelf life of unsterilized media will decrease due to the pH.

Q: You dilute 1 mL of bacterial culture with 99 mL of water.  What is the resulting dilution factor?

A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…

Q: based on the attached photo, compute the cfu/ml of 42 colonies at plate 10^5. show the complete…

A: To calculate the colony-forming unit per ml (cfu/ml) following formula can be used. CFU/ml= no. of…

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Q: Which is more Suitable for long-term storage: agar slant or plate? Explain why?

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Q: Could a MacConkey agar plate be used in place of the EMB plate in the Standard Analysis of Water?…

A: MacConkey agar is used as a differential and selective culture medium used in analysis of water…

Q: If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a…

A: Serial dilution  It is a method of stepwise dilution of a concentrated solution to get the desired…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.