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A Class Il aminoacyl-tRNA synthetase installs the formyl-methionine on tRNAMet in bacteria. A mutation at its active site leaves the synthetase fully functional except for one anomaly: the 3' end of the tRNA attacks the phosphorous atom in the aminoacyl-AMP intermediate instead of the carbonyl carbon. a) Please draw the complete aminoacylation of fMet to tRNAfet catalyzed by this mutant enzyme, starting from fMet+ATP+tRNAfMet and ending with the final product. Include the arrow pushing and the structures of amino acid and the sugar and phosphate groups within the adenylates. b) If one was to monitor incorporation of a phosphate group to the final product, which phosphate from what molecule should be isotopically labelled? c) If this tRNA synthetase was mutated to also accept tRNATP as a tRNA substrate (but no change to substrate specificy for fMet as the amino acid component), what would the translated amino acid chain be for the following mRNA sequence? 5' AUG AUC UGG GAG 3'

A Class Il aminoacyl-tRNA synthetase installs the formyl-methionine on tRNAMet in bacteria. A mutation at its active site leaves the synthetase fully functional except for one anomaly: the 3' end of the tRNA attacks the phosphorous atom in the aminoacyl-AMP intermediate instead of the carbonyl carbon. a) Please draw the complete aminoacylation of fMet to tRNAfet catalyzed by this mutant enzyme, starting from fMet+ATP+tRNAfMet and ending with the final product. Include the arrow pushing and the structures of amino acid and the sugar and phosphate groups within the adenylates. b) If one was to monitor incorporation of a phosphate group to the final product, which phosphate from what molecule should be isotopically labelled? c) If this tRNA synthetase was mutated to also accept tRNATP as a tRNA substrate (but no change to substrate specificy for fMet as the amino acid component), what would the translated amino acid chain be for the following mRNA sequence? 5' AUG AUC UGG GAG 3'

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A Class II aminoacyl-tRNA synthetase installs the formyl-methionine on tRNAfmet in bacteria. A mutation at its active site leaves the synthetase fully functional except for one anomaly: the 3′ end of the tRNA attacks the phosphorous atom in the aminoacyl-AMP intermediate instead of the carbonyl carbon. a) Please draw the complete aminoacylation of fMet to tRNA Met catalyzed by this mutant enzyme, starting from fMet+ATP+tRNAMet and ending with the final product. Include the arrow pushing and the structures of amino acid and the sugar and phosphate groups within the adenylates. b) If one was to monitor incorporation of a phosphate group to the final product, which phosphate from what molecule should be isotopically labelled? c) If this tRNA synthetase was mutated to also accept tRNATP as a tRNA substrate (but no change to substrate specificy for fMet as the amino acid component), what would the translated amino acid chain be for the following mRNA sequence? 5' AUG AUC UGG GAG 3'
Transcribed Image Text:A Class II aminoacyl-tRNA synthetase installs the formyl-methionine on tRNAfmet in bacteria. A mutation at its active site leaves the synthetase fully functional except for one anomaly: the 3′ end of the tRNA attacks the phosphorous atom in the aminoacyl-AMP intermediate instead of the carbonyl carbon. a) Please draw the complete aminoacylation of fMet to tRNA Met catalyzed by this mutant enzyme, starting from fMet+ATP+tRNAMet and ending with the final product. Include the arrow pushing and the structures of amino acid and the sugar and phosphate groups within the adenylates. b) If one was to monitor incorporation of a phosphate group to the final product, which phosphate from what molecule should be isotopically labelled? c) If this tRNA synthetase was mutated to also accept tRNATP as a tRNA substrate (but no change to substrate specificy for fMet as the amino acid component), what would the translated amino acid chain be for the following mRNA sequence? 5' AUG AUC UGG GAG 3'
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