With respect to DNA and RNA polymerases which statement is correct? OA) Only DNA polymerases are DNA template driven. OB) Only DNA polymerases require primers. OC) Only RNA polymerases are DNA template driven. OD) Only RNA polymerases require primers. E) Both require a DNA template, activated monomer units and a primer
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- In humans, the synthesis of Okazaki fragments requires: DNA polymerase III and DNA polymerase I DNA polymerase alpha (α) and DNA polymerase epsilon (ε) DNA polymerase delta (δ) and DNA polymerase epsilon (ε) DNA polymerase alpha (α) and DNA polymerase delta (δ) DNA polymerase alpha (α), DNA polymerase delta (δ), and DNA polymerase epsilon (ε)The smallpox virus, which actually has a fairly large genome size for a virus (about 190,000 base pairs), must use which of the following enzymes to replicate its nucleic acid? A. DNA-dependent-DNA-polymerase B. RNA-dependent-DNA-polymerase C. DNA-dependent-RNA-polymerase D. RNA-dependent-RNA-polymerase E. reverse transcriptaseWhat is the purpose and benefit of the polymerase chain reaction?
- What is the purpose of using DNA polymerase from Thermus aquaticus for the polymerase chain reaction rather than a DNA polymerase from a better-characterized bacterium such as E. coli?Which of the following processes of genetic information flow can occur under lab conditions, but has never been observed to occur under natural conditions (either in living cells or in viruses)? transcription of RNA from a DNA template (using DNA-dependent-RNA-polymerase) self-replication of RNA from an RNA template (using RNA-dependent-RNA-polymerase) direct-translation of protein from a DNA template (using special ribosomes) self-replication of DNA from a DNA template (using DNA-dependent-DNA-polymerase) translation of protein from an RNA template (using ordinary ribosomes)Why is the use of temperature-stable DNA polymerase animportant factor in the polymerase chain reaction?
- What is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic transformation should have?Compare DNA polymerase, RNA polymerase, poly(A) polymerase, and CCA-adding polymerase with respect to requirement for a primer, template, and substrates.In the Meselson Stahl experiment differentiating the possible modes of replication, how many rounds of replication were needed to determine that the following modes of replication were not used in E. coli? two rounds of replication for conservative; two rounds of replication for dispersive one round of replication for conservative; two rounds of replication for dispersive two rounds of replication for conservative; one round of replication for dispersive one round of replication for conservative; one round of replication for dispersive none of the above is correct
- What key structural features of the DNA molecule underlie its ability to be faithfully replicated?From the following DNA template, which sequence is synthesized by RNA Polymerase? 5’- T – C – C – T – A – A – G – A –3’ Group of answer choices 5’- A – G – G – A – T – T – C – T –3’ 5’- U – C – C – U – A – A – G – A –3’ 3’- A – G – G – A – U – U – C – U –5’ 3’- T – C – C – T – A – A – G – A –5’ 3’- T – G – G – A – T – T – C – T –5’In terms of new DNA strands that are generated, what are the differences between replication and conventional polymerase chain reactions?