A juice concentrate was colorimetrically assayed using Nelson’s test. One milliliter (1.00 mL) of the sample solution and various concentrations of the standard glucose solution were treated with freshly prepared Nelson’s reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbances at 480 nm for distilled water, glucose standard, and for the sample are 0.052, 1.702, and 0.926, respectively.
A juice concentrate was colorimetrically assayed using Nelson’s test. One milliliter (1.00 mL) of the sample solution and various concentrations of the standard glucose solution were treated with freshly prepared Nelson’s reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbances at 480 nm for distilled water, glucose standard, and for the sample are 0.052, 1.702, and 0.926, respectively.
Chapter89: Thin-layer Chromatography
Section: Chapter Questions
Problem 7P
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A juice concentrate was colorimetrically assayed using Nelson’s test. One milliliter (1.00 mL) of the sample solution and various concentrations of the standard glucose solution were treated with freshly prepared Nelson’s reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbances at 480 nm for distilled water, glucose standard, and for the sample are 0.052, 1.702, and 0.926, respectively. What is the reducing sugar concentration (mg/mL) in the juice concentrate? The equation of the line was plotted to be: y = 1.6656x - 0.0885
0.578 mg/mL |
||
577.9 mg/mL |
||
57.7 mg/mL |
||
5.78 mg/mL |
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