As part of a large scale study you are interested in determining the levels of Tubulin protein in different human cell types. You obtain multiple different cell types and intend to carry out an experiment. These cell types are A-431, Raji, MDA-MB-231, HCT-116, Hela, DU-145, PC-3 and Caco-2. These cell lines are derived from different tissues in the body. You hypothesize that MDA-MB-231 cells contain higher levels of Tubulin compared to the other cell lines 1. What type of gel electrophoresis will you use? Why? 2. You have at your disposal multiple different Tubulin antibodies. These are Rabbit anti human tubulin Rabbit anti mouse tubulin Human anti mouse tubulin Bovine anti Rabbit tubulin Donkey anti Chicken tubulin Which antibody would you pick for the experiment? Why?

As part of a large scale study you are interested in determining the levels of Tubulin protein in different human cell types. You obtain multiple different cell types and intend to carry out an experiment. These cell types are A-431, Raji, MDA-MB-231, HCT-116, Hela, DU-145, PC-3 and Caco-2. These cell lines are derived from different tissues in the body. You hypothesize that MDA-MB-231 cells contain higher levels of Tubulin compared to the other cell lines 1. What type of gel electrophoresis will you use? Why? 2. You have at your disposal multiple different Tubulin antibodies. These are Rabbit anti human tubulin Rabbit anti mouse tubulin Human anti mouse tubulin Bovine anti Rabbit tubulin Donkey anti Chicken tubulin Which antibody would you pick for the experiment? Why?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 20QP: If an extra nucleotide is inserted in the first exon of the beta globin gene, what effect will it...

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