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- Given an 8 M potassium chloride stock solution, explain whether it is possible to perform a dilution resulting in an 11 M working solution.You are supplied with the following: NaCl (Mr = 58.443 g/mol) 2.5 M Tris-Cl, pH 8 oplossing / solution (1 Litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10 % Sodium dodecyl sulfate solution Proteinase K solution (50 mg dissolved in 1 ml ddH2O)You need a digestion buffer consisting of the following: 15 mM NaCl 75 mM Tris-Cl, pH 8 16 mM EDTA, pH 8 0.8 % Sodium dodecyl sulfate 0.75 mg/ml proteinase KCalculate: How will you prepare 500 ml of the digestion buffer?To make up a solution of phosphate buffered saline (PBS), you need 10 mM Na2HPO4 (anhydrous) (FW: 141.96 g/mol), 3 M NaCl (FW: 58.44 g/mol), and 5mM KH2PO4 (FW: 136.09 g/mol). How many grams of each will you need to make up 850 mL of PBS?
- The doctor ordered Claforan 1400mg IM every 12 hours. The directions for the 2 gm vial state: Add 5 mL of diluent to yield an approximate concentration of 360mg/mL. How many mL will you give?TE buffer consists of 10 mM of Tris-Cl, pH 7.6 and 1mM of EDTA, pH 8.0. You need to prepare 3L of TE buffer and you have the following stock solutions: 500 mM Tris-Cl and 1 M EDTA. How will you prepare the 3L of TE? Write work clearly