A peptide was treated with Sanger's reagent followed by hydrolysis with 6M HCl for 24 hours at 105 oC. Chromatography of the mixture identified Val and Lys as the DNB labeled amino acids. Assuming a single peptide chain, explain how this could be and which is likely the actual N-terminal amino acid.
A peptide was treated with Sanger's reagent followed by hydrolysis with 6M HCl for 24 hours at 105 oC. Chromatography of the mixture identified Val and Lys as the DNB labeled amino acids. Assuming a single peptide chain, explain how this could be and which is likely the actual N-terminal amino acid.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A peptide was treated with Sanger's reagent followed by hydrolysis with 6M HCl for 24 hours at 105 oC. Chromatography of the mixture identified Val and Lys as the DNB labeled amino acids. Assuming a single peptide chain, explain how this could be and which is likely the actual N-terminal amino acid.
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