Do not give handwriting solution. A sample (1.90 x 102 mg) of an oligomeric protein of M, 113,000 was treatedwith an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightlyalkaline conditions until the chemical reaction was complete. The peptide bondsof the protein were then completely hydrolyzed by heating it with concentratedHCl. The hydrolysate was found to contain 2.85 mg of DNP-Val.2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids couldnot be found.Calculate the number of polypeptide chains in this protein. Give the answer as awhole number.number of polypeptide chains:3.9IncorrectO₂NDNP-ValNO₂NHH₂CCHCOOHHCH₂

DNP-Val : DNP-Val ( 2,4-dinitrophenyl-valine) is a derivative formed when the N-terminal amino acid…

Answered: A sample (1.90 x 10² mg) of an…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Can you describe the solubility of prolamin, a protein in rice protein bodies, in isopropyl or ethyl alcohol based on its molecular structure?
Based on molecular weights of purified (no beta-Me) and (beta-Me) sample, which are 140 kDa and 72 kDa, what can be said about the number and size of the protein monomers, as well as the nature of the bonds holding together the complete quaternary structure of the proteins?
Draw an ionization scheme for each of the amino acids Phenylalanine, Methionine, Tyrosine, Asparagine (from the most acidic form to the most basic form) , and show your computation for the pI/IP of each of the amino acids as well. Draw a complete structural representation for the tetrapeptide Phe-Met-Tyr-Asn (NOTE:use the zwitterion form) Give the IUPAC name for the tetrapeptide formation of F-M-Y-N. List all the possible tetrapeptide formed from one of each amino acids Phe-Met-Tyr-Asn? (NOTE: Use the three letter abbreviation code for the amino acids in answering D)
You have isolated the following peptide: His-Ser-Arg-Ala-Glu-Leu-Pro-Gly A) Calculate the approximate charge of this peptide at pH 1, pH 3, pH 5, pH8, pH 11, and pH 14. B) What is the PI of this peptide?
Following are two structural formulas for (S)-serine, one of the building blocks of proteins Is (S)-serine better represented by structural formula A or B?
A protein with a known mass of 240 kDa is treated with -mercaptoethanol, a sulfhydryl reducing agent. When the resultant peptide mixture was analyzed by SDS PAGE, one protein band of 60 kDa was present. How many disulfide bonds were present in the original structure of the protein? Group of answer choices -4 -2 -1 -3
Let’s consider histidine as a free amino acid in aqueous solution. a) Draw the most likely structure of histidine under biochemical standard state conditions. b) Given that free histidine has the following three pKa values, assign each to its corresponding acidic hydrogen or conjugate base in your structure from part a). pKa1 = 1.7; pKa2 = 6.2; and pKa3 = 9.1 c) For each pKa, give the corresponding expression for the equilibrium constant. It helps to write out the chemical equation for each. d) Create a speciation diagram for histidine by plotting Xi vs pH from pH = 4 to pH = 8 where Xi is the mole fraction of the two histidine species involved in the K2 equilibrium in part c).
For paper chromatography, the obtained Rf value of an amino acid with uncharged nonpolar R group is greater than the obtained Rf value of an amino acid with charged polar R goup. TRUE OR FALSE?
Ferritin is treated with SDS or SDS and b-mercaptoethanol and subjected to polyacrylamide gel electrophoresis. Only when very large amounts of bisacrylamide are added do you see two separate bands on the gel at relative migrations of 4.0245 and 3.9375 and the results are the same with and without b-mercaptoethanol. You decide to call these the light and heavy chains of ferritin. What are the actual sizes of the light and heavy chains of ferritin based on the SDS-PAGE data? What types of interactions hold these proteins together in the structure of ferritin?
For the following pentapeptides: Ser-Glu-Gly-His-Ala and Gly-His-Ala-Glu-Ser A. Compute their isoelectric pH (pI). Show full solution. Use standard pKa values. B. Do these peptides with the same amino acid composition have different net charges at pH 7.0? Explain briefly. C. Would you expect the titration curves of the two peptides to differ? Why or Why not?
A peptide was treated with Sanger's reagent followed by hydrolysis with 6M HCl for 24 hours at 105 oC. Chromatography of the mixture identified Val and Lys as the DNB labeled amino acids. Assuming a single peptide chain, explain how this could be and which is likely the actual N-terminal amino acid.
After staining an SDS-PAGE gel with Coomassie Blue G-250, the protein bands are visualized by de-staining the gel in a Coomassie Blue G-250 de-staining solution. This solution is made up of 10% acetic acid, 50% methanol, and 40% distilled water. How much of each of these components do you need to prepare 5 liters of Coomassie Blue G-250 de-staining solution?

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS