A sample of adenosine triphosphate (ATP) (MW 507, e 5 14,700 M-1cm-1 at 257 nm) is dissolved in 5.0 mL of buffer. A 250-mL aliquot is removed and placed in a 1 cm cuvette with sufficient buffer to give a total volume of 2.0 mL. The absorbance of the sample at 257 nm is 1.15. Calculate the weight of ATP in the original 5.0 mL sample.

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