A substrate is converted to a product via the reaction sequence (1) E + S ES k2 (2) ES +S ES2 k4 (3) ES2 , ES +P (4) ES , E +P (a). Using pseudo-steady state hypothesis for various forms of enzyme active sites, obtain relations between rates of individual steps in the mechanism. (b). Relating concentrations of vacant active sites to concentrations of occupied active sites, obtain expressions for the rate of consumption of S, (-Rs), and the rate of formation of P, Rp. (c). What are the maximum values of (-Rs) and Rp and under which conditions are these attained?
A substrate is converted to a product via the reaction sequence (1) E + S ES k2 (2) ES +S ES2 k4 (3) ES2 , ES +P (4) ES , E +P (a). Using pseudo-steady state hypothesis for various forms of enzyme active sites, obtain relations between rates of individual steps in the mechanism. (b). Relating concentrations of vacant active sites to concentrations of occupied active sites, obtain expressions for the rate of consumption of S, (-Rs), and the rate of formation of P, Rp. (c). What are the maximum values of (-Rs) and Rp and under which conditions are these attained?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:A substrate is converted to a product via the reaction sequence
(1) E +S ES
k2
(2) ES + S ES2
k4
(3) ES2
ES +P
(4) ES , E +P
(a). Using pseudo-steady state hypothesis for various forms of enzyme active sites, obtain relations
between rates of individual steps in the mechanism.
(b). Relating concentrations of vacant active sites to concentrations of occupied active sites, obtain
expressions for the rate of consumption of S, (-Rs), and the rate of formation of P, Rp.
(c). What are the maximum values of (-Rs) and Rp and under which conditions are these attained?
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