A team of R&D scientists were trying to make a "better" enzyme. A molecular biologist mutated ami residues of an existing enzyme. An enzymologist on the team evaluated the engineered enzymes by m the dissociation constants between a substrate-analog and the different engineered enzymes. The engir measured Vmax of each enzyme, using a natural substrate of the enzyme. Which engineered enzyme is and why? Provide a thermodynamic explanation. Include a reaction-progress energy diagram in your Engineered Enzyme ID KA (uM) Vmax (uM min!) A 10 150

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