a. If the mixture is dabbed onto chromatography paper that was then placed into a polar solvent, rank the R, values for each component of the mixture from high to low after the solvent has saturated the paper. Justify your answer. b. If the mixture is poured into a chromatography column and then eluted with a very nonpolar substance, which component of the mixture would leave the column first, and why?

a. If the mixture is dabbed onto chromatography paper that was then placed into a polar solvent, rank the R, values for each component of the mixture from high to low after the solvent has saturated the paper. Justify your answer. b. If the mixture is poured into a chromatography column and then eluted with a very nonpolar substance, which component of the mixture would leave the column first, and why?

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter88: Column Chromatography

Section: Chapter Questions

Problem 4P

See similar textbooks

Similar questions

Fully explain what the data in IR and GC-MS spectra mean in the dehydration of 2-Methylcyclohexanol . GC-MS data (gas chromatography): Illustrated in picture *ignore peak at 8.87 minutes Peak 1 mass = 96.2 (1-methylcyclohexene) Peak 2 mass = 96.2 (3-methylcyclohexene) Peak 3 mass = 114.2 (impurity) IR Spectra (Infrared Spectroscopy): Illustrated in picture.
The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67
Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram? Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?
Z and E -oct-3-en-2-ol is hard or easy to separate in thin layers in chromatography?
Which of the following carotenoid structures below would you expect to have the lowest RF in a chromatography experiment using an ether-petroleum ether mobile phase? Why? Lycopene: β-carotene: Vitamin A
A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?
A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most soluble in the mobile phase and why?
1. What is the retention time of molecule 9?2. What happens to the peak area if the sample amount is doubled?3. If run time is stopped at 5.5 mins, will molecules 8 & 6 be separated and quantified?4. If the flow rate was doubled, will 8&6 be separated and quantified?5. Does a single peak at time T in the chromatogram indicate only one compound is eluted?
Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.
If a 0.0920 g sample of a 1:1 mixture of 4-nitroaniline and 2-nitroaniline was separated by column chromatography, and the recovered fractions weighed 0.0191 g ( 4-nitroaniline) and 0.0200 g (2-nitroaniline), what was the percent recovery of these two isomers? 20.6% 43.5% 21.7% 42.4%
How complexation of diclofenac sodium with hydroxy propyl beta-cyclodextrin by high pressure liquid chromatographic (HPLC) method can enhance the solubility of diclofenac sodium.
A thin layer chromatography experiment is planned to determine the Rf values of four compounds. List the following compounds in order of increasing Rf values from shortest to longest. (If B is shortest, then A, C, and D is longest, enter BACD )