Answered: 1. An undercooked boiled egg was…

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter1: Introduction To Microbiology

Section: Chapter Questions

Problem 1UTM: The Centers for Medicare and Medicaid Services (CMS) have initiated revised policies regarding...

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1. An undercooked boiled egg was received in the microbiology laboratory with a request culture and identification. This egg was served in a pack lunch during a birthday party. All guests who ate this egg were brought to the hospital for vomiting and dehydration. Some had bouts of diarrhea. The signs and symptoms were short-lived after treatment was received. The egg was homogenized in tryptic soy broth. This was plated in sheep’s blood agar plate and MacConkey agar plate. After 24 hours of incubation, the following observations were noted:

Sheep’s blood agar plate: 4+ (many) beta hemolysis, creamy white colonies
MacConkey agar plate: no growth
The gram stain result of the isolate yielded gram-positive cocci, mostly in clusters.

If Staphylococcus aureus is suspected, list 3 tests that will be most relevant in its identification – i.e. to differentiate it from other gram-positive cocci

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