After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?
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After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?
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- CCCP is observed to have caused increase the SDH activity in DCIP assay. Explain why?Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?IN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR ABSORBANCE. IS IT POSSIBLE?
- What is an ONPG test? WHat is he principle behind this assay.If you mix two unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is used.For tha analysis of a specific drug present in different pharmaceutical preparations, explain when is more appropriate to use absorbtion based assay and when is more appropriate to use a fluorescence based assay.
- What is the difference between in-ovo and ex-ovo types of CAM Assay?is bradford assay using dye Coomassie Brilliant Blue G-250 a different method from using a spectrophotometer ? if so how is it different to determine protein concentration?What reaction would you expect when performing a positive control in the catalase assay? What would it mean if a known catalase-positive bacteria did not produce the expected reaction?
- Is there any way to stabilize color after adding stop solution in ELISA in Which HRP and TMB used as enzyme and chromophore?Why is it essential to add sodium azide to succinate dehydrogenase assay solution? What is the purpose of including the sodium succinate solution?How would you determine the concentration of a protein that falls outside (above or below) the linear range of this assay? asap