amino glycine pKa is 2.4, 9.8 Calculate the most effective buffering range. if there is two pka, since there are 2 buffer region, is the buffering range 1.4~3.4 and 8.8~10.8? i would like to know the correct answer and detailed solution/reason.
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- Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…Calculate the pH of a mixture of 0.25 M acetic acid and 0.20 M sodium acetate. The pKa of acetic acid is 4.76.Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.
- 1.2. Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Cys, His and Leu Starting pH: _____ Ending pH: _____Calculate pH of buffer prepared by mixing; 10ml of 0.10M of CH3COOH and 20ml of 0.10M of CH3COONa.If you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?
- Using the equation for the best-fit straight line through your data, the average absorbance of your unknown samples U1 and U2, and any dilution factors (DON’T LEAVE OUT THE DILUTION FACTOR), calculate the concentration of protein in the original unknown protein sample. y = 1.6849x + 0.0414R² = 0.9904Calculate the pH of a buffer that contains 0.15 M MOPS anionic form and 0.25M MOPS zwitterion. The pKa is 7.2Draw titration curve for compound X when NaOH equivalence(s) is/are added. Indicate the buffering region(s) with a box.
- determine the pKa of the acid and ph of the buffer sol'n of a buffer mixed with 0.1 M tris and 0.5 M chloine salt having dissociation constant of 1.072x10^-6.if you had a protein sample solution of unknown concentration which gave an absorbance of 0.992 after the two-fold dilution (25 μL water + 25 μL sample). If the standard curve we constructed. what would you need to do to your sample in order to find its protein concentration more accurately?Describe the preparation of 2.00 L of 0.100 M glycine buffer, pH 9.0, from glycine and 1.00 M NaOH. What mass of glycine is required, and what volume of 1.00 NaOH is required? The appropriate pKa of glycine is 9.6