A glucosamine solution of unknown concentration was diluted as follows:  1 mL of the solution was diluted to 5.0  mL.  A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank).    What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL? Given: Corrected absorbance = 0.395 y= 1.165x+0.055 Standard equation

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 8SST
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A glucosamine solution of unknown concentration was diluted as follows:  1 mL of the solution was diluted to 5.0  mL.  A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank).   

What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL?

Given:

Corrected absorbance = 0.395

y= 1.165x+0.055 Standard equation 

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