An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments is tabulated below. Glycogen (mM) Product Formed Glycogen only (mM/min) Product Formed Glycogen and Inhibitor (mM/min) 0.5 22.6 15.9 1.0 32.1 24.9 1.5 38.1 28.2 2.0 41.2 35.1 2.5 44.7 40.0 3.0 48.5 43.3   Draw the Michaelis-Menten and Lineweaver-Burke plots of these dat In the attachment.   Determine the form of inhibition observed from these results, and explain your rationale for this form.   Determine the values for Km and Vmax from these results

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  1. An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments is tabulated below.

Glycogen (mM)

Product Formed Glycogen only (mM/min)

Product Formed Glycogen and Inhibitor (mM/min)

0.5

22.6

15.9

1.0

32.1

24.9

1.5

38.1

28.2

2.0

41.2

35.1

2.5

44.7

40.0

3.0

48.5

43.3

 

  1. Draw the Michaelis-Menten and Lineweaver-Burke plots of these dat

In the attachment.

 

  1. Determine the form of inhibition observed from these results, and explain your rationale for this form.

 

  1. Determine the values for Km and Vmax from these results.
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