For an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h, calculate g, n, and k forthis culture.

Q: starting with four bacterial cells per milliliter in a rich nutrient medium, with a 1-hour lag phase…

A: Bacterial Growth --Bacterial Cell growth is proliferation of bacterium in to daughter cells ,…

Q: A specific heat treatment used on a particular bacterial suspension has a DRT of 10 seconds, how…

A: Heat treatments have been used for the reduction of bacterial numbers and even for sterilization…

Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…

A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…

Q: Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled…

A: Agar or agar-agar is a jelly-like substances obtained from red algae. Agar is a solidifying agent…

Q: Wintrobe from Westergren

A: Wintrobe test and Westergren test are both used for ESR. ESR is erythrocyte sedimentation rate. It…

Q: Calculate mean growth rate and generation time of a culture that increase in exponential phase from…

A: Generation time is time taken for culture organisms to double in size. Generation time = t/n  where…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features…

A: Staphylococcus aureus and Streptococcus pyogenes are gram positive bacteria that cause infections in…

Q: in 5 hours, your bacterial culture went from a concentration of ~105 CFU/mL to ~1.6 x 106 CFU/mL,…

A: n=1.6x106 CFU/ml N=(no of cells at the beginning )=105 CFU/ml n=Nx2x x=no. of generation…

Q: Calculate the growth rate and generation time of a culture that increases in the exponential phase…

A: The growth of bacteria over a period of time is represented by a growth curve. This curve tells the…

Q: Explain why you can use OD675 (optical density at a wavelength of 675nm) as a proxy for cell density…

A: Algae are group of predominately aquatic, photosynthetic, and nucleus bearing organism lacking true…

Q: How to calculate for FAME yield given the dried FAME extracted of 0.021 g and the volume of culture…

A: Fatty acid methyl esters or FAME are a type of fatty acid esters. This is formed by the…

Q: What is Zeta Sedimentation Ratio?

A: As per our answering policy, we can only answer one question at a time. Please post the other…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: Provided with the following data, compute the corresponding CFU/ml of the original culture. ssume…

A: Ideally, plates with colony 25-250 colonies are used. As above 250 colonies are too numerous to…

Q: What medium is this? What is the result in tube at left? In second tube? What does it tell you about…

A: One stab towards the bottom of the SIM tube is used to inoculate it. If the microorganism is motile,…

Q: Why does turbidity lose reliability at high cell concentrations when the culture reaches the…

A: The turbidity measurement method for counting the cells in the tissue culture is the estimation and…

Q: Calculate the colony forming units (CFU) if 100  colonies grow in agar using  a 0.01-mL calibrated…

A: One colony equals to 100 col/mL in  0.01 -mL calibrated loop.

Q: Calculate the concentration of the overnight stock culture in CFU/mL

A: Colony-Forming Units (CFUs) refer to the number of individual colonies of any microorganism that…

Q: Wintrobe from Westergreen regarding accuracy of the test in a tabulated order.

A: Both methods are used for estimating E.S.R rate or the erythrocyte sedimentation rate. Most of the…

Q: Are you able to identify the lag, log, stationary and death phase in using this growth curve?

A: Microbes grown in a closed or batch culture in which  certain conditions are that no nutrients are…

Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

A: Introduction: A CFU stands for colony-forming units. It is a unit that we use for estimating the…

Q: Compute for the dilution of the bottle and each tube. Refer to the schematic diagram below:…

A: When a concentrated solution is made less concentrated that it is said to be diluted. The…

Q: Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4 7H2O, 0.5; K2HPO4, 1; KCl, 0.5…

A: Culture media is proper nutrient for the growth of the microorganisms, which help to grow a variety…

Q: Explain why the following steps are necessary during subculturing:  Cooling the inoculating loop…

A: Subculture is the process of the removal of the media and transferring the cells from previous…

Q: If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock…

A: Given: volume of stock solution is 15 mg/100 ml Needed amount is 1 mg kinetin in 25 ml Volume of…

Q: Where do you expect to see the highest number of CFU on a plate: Select one: a. When pasteurized…

A: The number of colony forming units on the countable plate is multiplied by 1/FDF to get the number…

Q: Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium…

A: Bacteria are organisms that are found almost everywhere on earth and are free-living cells that are…

Q: WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE…

A: Indole test is a biochemical test for distinguishing between Enterobacter aerogenes and Escherichia…

Q: You were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent.…

A: The dilution factor can be defined as the ratio of Volume of the initial solution to the volume of…

Q: What organism is the current record holder for theupper temperature limit for growth?

A: A thermophile is an organism that thrives at reasonably high temperatures, between 106 and 252 ° F…

Q: In determination of microbial motility, what is the value of a hanging-drop perparation? what is the…

A: Hanging drop is a motility testing which helps microbiologist to view motility of bacterium.

Q: The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Q: Which B. megaterium culture, a culture in the exponential growth phase or a culture in the…

A: Ultraviolet radiation is a short-wavelength, non-ionizing radiation, and its wavelength ranges from…

Q: A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it.…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Q: Calculate the doubling time of a culture of E. coli that is started with 3x105 CFU/mL and grows to…

A: Given: No = 3x105 CFU/mL (number of bacteria at the beginning of a time interval) Nt = 4.6x108…

Q: 1) Write your dilutions as a rational number AND in scientific format? What was your total dilution…

A: Serial dilution is a process in which a sample is taken and diluted through a series of standard…

Q: use these OD numbers to plot growth curve for E. coli K12– and estimate generation time for this…

A: The growth curve for E.coli K12 is plotted as follows:

Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…

A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…

Q: You have a starter culture containing 8 x 109 cells per mL, from which you take 10 mL to inoculate a…

A: The generation time of culture is considered to be the time at which it doubles and the mean growth…

Q: based on the attached photo, compute the cfu/ml of 42 colonies at plate 10^5. show the complete…

A: To calculate the colony-forming unit per ml (cfu/ml) following formula can be used. CFU/ml= no. of…

Q: The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula…

A: Original cell density (OCD) can be found using the number of colonies of colony-forming units,…

Q: You are cultivating Escherichia coli in a chemostat culture. The maximum specific growth rate (µmax)…

A: A chemostat is a bioreactor containing culture where the fresh medium is continuously introduced at…

Q: Which is more Suitable for long-term storage: agar slant or plate? Explain why?

A: In order to isolate the bacteria from samples, they need to be inoculated into a growth medium which…

Q: Calculate the doubling time (td) and specific growth rate (μ) using the logarithmic growth stage of…

A: The exponential phase or the log phase or the logarithmic in the process of bacterial growth is…

Q: You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells…

A: To count no. Of cells/ml using hemocytometer, count the number of cells in all four outer squares…

Q: You have found that the D-value (decimal reduction value) of an antimicrobial agent to be 4 minutes…

A: In sterilization techniques, D-value or decimal-reduction value refers to the time taken, under…

Q: Are you able to identify the lag, log, stationary and death phase in using this growth curve? Are…

A: The growth curve of bacterial cells can be divided into four distinct phases known as lag, log (or…

Q: If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a…

A: Serial dilution  It is a method of stepwise dilution of a concentrated solution to get the desired…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.