Assuming you have an agonist, a competitive antagonist, and an easy assay for measuring binding of the agonist, describe a simple experiment that would allow you to determine specific binding, nonspecific binding, and total binding of the agonist at a SINGLE concentration.

Assuming you have an agonist, a competitive antagonist, and an easy assay for measuring binding of the agonist, describe a simple experiment that would allow you to determine specific binding, nonspecific binding, and total binding of the agonist at a SINGLE concentration.

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter6: Cell Communication

Section6.3: Reception

Problem 2C

See similar textbooks

Related questions

Q: Draw the chair conformation of B-D-Altropyranose given the structure of D- Altrose. (Upload your ans...

A: Chair conformation is the most stable structure of a cyclohexane ring. in the chair conformation, th...

Q: Using Molisch's reagent, an unknown carbohydrate solution was found to be positive (+). Within three...

A: Molisch's test is done for detection of carbohydrate. Barfoed's test is used for detection of monosa...

Q: How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solution

A: Proteinase K is a serine proteinase enzyme used to digest proteins. It is produced from a mold(fungu...

Q: 10 samples of genetically modified organisms ( Animals ) With brief descriptions of each

A: Genetically modified organisms (GMOs) are those organisms (plants, animals or microorganisms) whose ...

Q: B- what different between organic compound and inorganic compound ? with two example for each one. *

A: The level of organization of the human body from lowest to highest level includes atoms, molecules, ...

Q: According to the configuration of the isostatic, syndiotactic and atactic polymers. Define each of ...

A: TACTICITY :- The steric arrangement in polymers is called tacticity.

Q: In moelcular genetics, initiation is often accomplished using proteins that prevent elongation. Nam...

A: Initiation of a process in genetics is an important event. At this stage, cell prepare itself for th...

Q: Why can't humans digest cellulose while ruminants can? What is the difference between the two organi...

A: Cattle, sheep, and goats are examples of ruminant livestock. Ruminants are hoofed mammals with a dig...

Q: A small peptide has two pKa values of 3.42 and 8.74. What is the isoelectric point for this peptide?...

A: Isoelectric point: The isoelectric point(pI) is the pH at which particular molecule carries no net...

Q: In one sentence each, how are the following bonds formed and broken in biomolecules? a) ester bond...

A: There are many types of covalent bond common in biomolecules. C-C bonds are basic to all organic mol...

Q: In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used i...

A: UV/Visible Spectrophotometry detects the absorbance of light in the UV/Visible range of the electrom...

Q: How do D-amino acids differ from L-amino acids and what are the biological roles that are played by ...

A: Amino acids are monomers of protein they are linked with each other by forming peptide bo...

Q: Briefly explain the Michaelis-Menten model of enzyme kinetics.

A: Enzymes are commonly composed of protein molecules that catalyze the biochemical reaction by decreas...

Q: Below is the primary sequence of a viral protein. MSVVNTEIKFPTHLRSGDFAIIDGMVVEVTSVEYKPVEQAVYLKYRYHL...

A: Biomolecules are organic molecules made up of mainly carbon and hydrogen but there are other element...

Q: What is the one ingredient which turns up in all of the formulas and where does it come from? How an...

A: Tetrodotoxin: Tetrodotoxin is a low-molecular-weight neurotoxin which is a wide variety of taxa. It...

Q: Define phosphodiesterase

A: Proteins are macromolecules made up of L-amino acids. Amino acids when combined with a peptide bond,...

Q: Protein folding

A: proteins are composed of some 20 types of amino acid residues, and they would be more or less alike ...

Q: Why can't we store Vitamin C in our bodies?

A: Vitamin C- Ascorbic acid It is vital for tissue repair , collagen synthesis and it's deficiency- cau...

Q: Experiment No. 2 AMINO ACIDS AND PROTEINS Data Solubility in water Sample Solubility (So...

A: Disclaimer: "Since you have posted a question with multiple subparts, we will solve the first three ...

Q: Kingdom Type of Cells Number of Cells How it Obtains Reproduction Food Asexual by binary fission, bu...

A: Eukaryotes are organisms whose cells contain a nucleus (along with other membrane-bound organelles. ...

Q: What group is added to modify methionine on the initiator tRNA in bacteria O phenyl O formyl O methy...

A: Initiator tRNAs (tRNAi) transport methionine (or its product, formyl-methionine) to ribosomes, where...

Q: At a pH of 7.40, the carbonic acid ratio is, a. 35:1 b. 4:1 C. 20:1 d. 3:1

A: Given Values: pH = 7.4 pKa = 6.1

Q: Q21. What regulators of gene expression bind the lac promoter region if E. coli is grown in media co...

A: In the absence of lactose the repressor binds to the operator sequence adjacent to the promoter and ...

Q: Connection between Electron Transport & Phosphorylation Task: 1. Define P/O ratio 2. Explain its...

A: Our body is always working, continuously doing various metabolic activities even when we are sleepin...

Q: Draw the biosynthetic pathway with explanations of each step for the following secondary metabolite ...

A: Polyketides are naturally found molecules of a large and diverse group of secondary metabolites whic...

Q: 2) A group of three researchers (who often tend to disagree with one another) isolated a new protein...

A: a) This must be enzyme containing a iron- sulphur cluster not a Mn- cluster.Iron- sulphur cluster co...

Q: Draw the Micelle generated when the soap is dissolved in water and the heater. For this representati...

Q: Functional groups and linkages assignment Complete the reactions below. Circle and identify all func...

A: The hydrocarbon compound is N-butylpropanamide which is reacting with water.

Q: What is ENDOSYMBIOTIC THEORY

A: All living things are made up of cells which are of two basic types: prokaryotes or eukaryotes. Prok...

Q: When you are served with the dessert, you had an emergency call from home and you need to return ho...

A: From the production of food to consumption of food it goes through several steps which include m...

Q: What is MRI? Why do doctors order an MRI scan? What are the do’s and don’ts when undergoing an MRI...

Q: How do you prepare 42:1 chloroform/isoamyl alcohol 70% ethanol?

A: Preparation of 42:1 Chloroform / Isoamyl alcohol Add 42ml of Chloroform and later add 1ml of Isoamy...

Q: If one DNA segment has the following base composition, 5'-CAGTTAGTCA-3', which of the following seq...

A: DNA universally has 4 N-bases: Adenine, Thymine, Guanine and Cytosine If the given strand sequence o...

Q: In a sample dsDNA from an organism, the amount of thymine is analyzed to be 12 μmoles when the A+T/G...

A: In double stranded DNA the composition of bases follows the rule: A+T= G+C.

Q: 1. Would you say that indicator tests are qualitative or quantitative? Explain your answer.

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any ...

Q: Which of the following peptides will likely form a loop or turn in a protein and explain why

A: The turns are the third type of secondary structure. These are short, U-shaped secondary structures,...

Q: Do cultivated plants suffer from diseases just like humans?

A: All living beings have cells, which are the most basic fundamental unit of life. Cells are responsib...

Q: What are the advantages of the presence of organelles in eukaryotic cells?

A: The eukaryotic cell has several organelles and is compartmentalized.

Q: For each of the following genotypes, explain how mutation (identified by a (-) will affect the organ...

Q: 3. While the binding of Coomassie blue to proteins can lead to accurate measurements of protein conc...

A: Proteins absorb strongly at 280 nm due to the presence of aromatic amino acids (Phenyl alanine, tyro...

Q: How many different chemical units make up a nucleoside? (А) 5 в) 2 c) 4 D) 3

A: We are authorized to answer one question at a time, since you have not mentioned which question you ...

Q: Which functional site is found in the small subunit of the ribosome? peptidyl transferase centre O d...

A: Ribosomes, huge ribonucleoprotein particles with three RNA molecules and more than 50 proteins, are ...

Q: 26. Dietary triglycerides are hydrolyzed by pancreatic lipase, which is related to but distinct from...

A: The pancreas is a heterocrine gland because it secretes digestive juices and hormones. Pancreatic am...

Q: A newly discovered blob protein folds very rapidly in the presence of protein disulphide isomerase ...

A: Proteins are polypeptide structures consisting of one or more long-chain amino acid residues. They a...

Q: Sam does not consume alcohol, while his brother Sebastian regularly drinks large quantities of alcoh...

A: Liver, the largest gland in the human body has various secretory and metabolic functions. It secrete...

Q: Describe the types of pathogens. Give an example of an obligate pathogen, a facultative saprophyte, ...

A: Microorganisms, often known as microbes, are microscopic organisms that can be found all over the pl...

Q: How many different components make up a nucleoside? 4 2 3 5

A: Nucleosides are glycosylamines that are phosphorylated to form nucleotides.

Q: I. Name the following carbohydrates using the proper way of naming/IUPAC system. a. Erythrose b. Lyx...

A: Carbohydrates are the primary source of energy for the body. Glucose is the simplest form of carbohy...

Q: Discuss the role of mould in food process industry and analyze the process of one food product that ...

A: Role of mould in food process industry- 1. Moulds are basically a fungus that are used in the prepa...

Q: Each of the following statements concerning mitochondria is true, EXCEPT a.The mitochondria require...

Question

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Agonist, Competitive antagonist, Specific & Non-specific binding

VIEW

Experiment to determine Total, Specific and Non-specific binding of agonist

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

Using the cultured cells used in this experiment and described in the introduction, how would you approach determining the potency of an agonist to the D2 receptor?
In experiment，how would you approach determining the potency of an agonist to the D2 receptor？
To maximize the activity of an α4β2 nicotinic Achtylchcholine Receptor (nAChR), how many agonist binding sitea should be occupied?
what is the difference between a partial agonist,full agonist and an inverse agonist ,and why they will be difference?
The EC50 of an agonist for a GPCR is almost always (greater than, equal to, or less than) the KD of the same agonist at the same GPCR. Why?
Estimate the binding affi nity of a ligand for its receptor from the followingdata:
The potency of agonists is frequently defined in terms of their affinity and theirefficacy. Explain what these two terms mean. Use diagrams to illustrate your answer
Inhibitors of acetylcholinesterase, such as edrophonium, are used to treat Alzheimer’s disease. The substrate for acetylcholinesterase is acetylcholine. Structures are attached. What kind of inhibitor is edrophonium? Explain. Can inhibition by edrophonium be overcome in vitro by increasing the substrate concentration? Explain. Does this inhibitor bind reversibly or irreversibly to the enzyme? Explain.
Briefly explain the difference between a receptor antagonist and a receptor inverse agonist. Describe both the resulting receptor conformation as well as the signal transduction.
what would the Pharmacokinetics and Pharmacodynamics be of an hullicinate drug like LSD that acts as an agonist?
The NMDA receptor I/V curve is nonlinear, why? What about the GluA2-containing AMPA receptor I/V curve? Draw both under normal physiological conditions. In addition, explain what happens for the NMDA receptor I/V curve when you lower the magnesium concentration.
Which arrestin is required for activation of phosphorylation of MDM2 during chronic Iso stimulation? use the following Figures 1and 2 respectively to explain please