You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is the dilution factor?

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A: "Microbiology" is the study of microorganisms and associated topics. Microbiology has gone a long…

Q: If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original…

A: CFU stands for colony-forming unit that gives rise to a particular colony. It is generally assumed…

Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: If you perform the motility test on Motility Medium and hanging drop for Bacillus cereus and B.…

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Q: to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is…

A: Motility is the ability of an organism to move from one place to other, generally, the bacteria…

Q: What is a pure culture?

A: Introduction  Culture is referred to th process of growing the cell outside the animal or plant body…

Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…

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Q: If any of the water sample plates had a lawn or too many colonies to count, what might you have…

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Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?

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Q: What concentration of agar is used in a semisolid medium for motility determination?

A: Motility is a feature characteristic of only some bacteria. This ability allows the bacteria to move…

Q: If you run the motility test on Motility Medium and hanging drop for Bacillus cereus and B.…

A: Answer:  HANGING DROP TECHNIQUE : It is the special type of wet mount which is made from a drop of…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

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Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

A: The following are needed for calculating the dilution factor  1) Original volume of  solution we…

Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…

A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…

Q: yield

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Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

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Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…

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Q: What side of the electrophoresis chamber (red or black) should the wells be on when the gel is…

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Q: temperature and light source affect the number and type of colonies that can be found in the…

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Q: How is the correct way of incubating culture plate media? Explain why is that so.

A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…

Q: Why do we use the term CFU (colony forming unit) instead of reporting bacteria per ml? Explain.

A: The  bacterial species are responsible for causing several diseases within the human body. So, for…

Q: You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the…

A: Estimation of the number of viable cells is important in microbiology protocols. It is estimated…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-° dilution to a sterile petri…

A: Dilution is a process by which solution (water or diluents) are added to the solvent (main solution)…

Q: Heat-labile material to be introduced to culture medium was filtered using membrane filters with…

A: given that the heat labile material which is introduced to culture media is filtered using a…

Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

A: Introduction: A CFU stands for colony-forming units. It is a unit that we use for estimating the…

Q: Why is the incubation temperature criticalwhen using the Filter Method when testing water samples?

A: Incubation temperature is critical for bacterial growth if this temperature is too high or too low…

Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…

A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…

Q: You were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent.…

A: The dilution factor can be defined as the ratio of Volume of the initial solution to the volume of…

Q: why In spread-plate technique, the bacterial suspension volume  should not exceed 0.1ml.

A: Spread plate method is a technique which uses a Petri plate or dish in which a growth medium or gar…

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

Q: A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it.…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Q: When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies…

A: Dilution is the process of decreasing the concentration of the solution. The cells count decreases…

Q: Assuming you had a bacterial culture with a concentratoion of 2x10^9 bacteria/mL, whaat volume would…

A: A microbiological culture, also known as a microbial culture, is a technique for growing…

Q: If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40…

A: In the question it is asked that they want to plate out 40 bacteria, and this count comes under the…

Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…

A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…

Q: You have a starter culture containing 8 x 109 cells per mL, from which you take 10 mL to inoculate a…

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Q: Why should your solvent be evaporated from the sample placed on the sterile disk before you place it…

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Q: You dilute 1 mL of bacterial culture with 99 mL of water.  What is the resulting dilution factor?

A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…

Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. You then…

A: A serial dilution is any dilution in which the concentration is decreased by the same factor in each…

Q: You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show…

A: Introduction :- A test to determine whether you have a bacterial infection is a bacterium culture.…

Q: What is the meaning of the word sterile? What would happen if culture media freshly prepared were…

A: Sterile It refers to the state of being free from any bacteria or any other living microorganisms.…

Q: In preparing bacterial smear, why do we need to pass the slide over the flame?

A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…

Q: In the conduct of serial dilution, which of the following tools is NOT necessary?   a. Agar…

A:

Q: If you were using the quadrant streak plate method to plate a very dilute broth culture (with many…

A: Streak plate technique is the technique or method that is widely used for the isolation of a pure…

Q: Why should agar media be completely dissolved before they are dispensed in tubes and plates? What…

A: Introduction  A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: a sample was diluted by placing a 0.1 mL of dilutent and 1 mL of this dilution was pour-plated.…

A: The CFU/ml in the Original Culture

Q: If you plated 0.1mL of a 103 dilution and you obtained 235 colonies on your agar plate, how many…

A: Introduction: CFU's: Colony-forming units are used to estimate the concentration of microorganisms…

Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…

A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml

Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…

A: Culture consists of appropriate substances which provides the growth to the desired organisms.

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.