You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. You then repeat this procedure two more times. What is the final dilution factor of this serial dilution? O 1) 10-4 O 2) 102 O 3) 10-6 4) 10-2 O 5) 104
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- You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor?How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar plate, what is the final dilution?
- Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix. You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water. What is the dilution of the bacterial culture in bottle B?In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?
- What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?What is this test? What coated the bottom of the wells? What were we testing for? How did we know it was positive? How did we know the test was valid? How did we stop the reaction? How did you know you did a good job micropipetting your samples?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?