Below is the genotyping results using PCR and gel electrophoresis for a DNA-binding regulatory transcription factor called TF111. Write down the genotypes for normal wild type, skin tumor B, and skin tumor C. DNA Wild type Skin tumor Skin tumor ladder control Cell type B Cell type C 1,000bp 900bp wild type TF111 allele 700bp 500bp 300bp 200bp 40bp mutant TF111 allele Primer dimer Genotype for Wild type control: Genotype for skin tumor cell type B: Genotype for skin tumor cell type C:
Q: A mutant monkey was identified that showed resistance to a viral disease 'Kansah'. The kan gene,…
A: In molecular biology, PCR (polymerase chain reaction) plays an important role. With the help of this…
Q: Describe the following techniques for determining gene or protein function with advantages…
A:
Q: Rewrite the following sentences after correction. Indirect ELISA can detect polygenic gene…
A: ELISA is enzyme linked immunosorbent assay, which is an immunosorbent assay designed for detecting…
Q: the best technique to answer the following question: Does GAL4 transcription activator bind to GAL3…
A: We can detect GAL4 transcription activator binds to GAL3 co-repressor via Bacterial two-hybrid…
Q: The drawing below depicts a restriction map of a segment of a human chromosome. The cut sites shown…
A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. If you need help with other sub…
Q: Which of the examples of genetic testing below are prognostic tests? Which are diagnostic? (a)…
A: Genetic testing identifies changes in proteins, genes, or alleles, chromosomes. prognostic test…
Q: ABOUT Phenylketonuria Explain Potential technical issues and limitations of PCR technology are…
A: Phenylketonuria (PKU) is a metabolic disorder in which the amino acid phenylalanine is not properly…
Q: Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains…
A: Sickle cell anemia is a result of a mutation in the HBB gene. This gene contains the restriction…
Q: DATA Tables for Creature Gene Analysis: GENE A (Hair) GENE (Skin color) DNA CAA GGA TAT DNA GGA CGC…
A: DNA GGA CGC CGA mRNA GGA CGC CGA Amino acids Glycine (Gly), Arginine(Arg),…
Q: Virtually all steps of embryological development depend on: a. Alterations in the genome b. Tumor…
A: Embryological development is the growth and development of embryo by cell division and cell…
Q: 3' c |c 5' 5' 1 1 1 A T 2 2 2 A C c 3 3 3 G T 4 4 4 A Reference - DMD - Template 5 6 T C c G…
A: Coding strand Coding strand is- GGT AAT GGC AAT AGA GGC CGA TAC ACA GGA patients…
Q: 1 [Choose ] Choose Promoter Operator Regulatory Gene Active Repressor Protein Structural Genes 3…
A: Lac operon is basically a mechanism essential in the metabolism in the E.coli bacteria.
Q: What are the key findings of cancer genomics studies as they relate to chromatin biology?
A: Genomics is a branch of biology, which deals with studying and analyzing the structure, evolution,…
Q: The image at right shows (A) RNA blot analysis (hsp1) and (B) protein abundance (HSP, Actin) for…
A: Heat shock proteins Heat shock proteins are the molecular chaperones that help in refolding of…
Q: Which statement is right the polyclonal antibody is expensive to produce the monoclonal antibody…
A: Antibodies are giant Y-shaped proteins known as immunoglobulins that are created by B cells as a…
Q: Meta analysis C9orf72 Known Novel • 20 15 10 SARM1UNC13A МОВР GPX3-TNIP1 -LOC101927815- SCFD1- 5 CO…
A: ALS (Amyotrophic Lateral Sclerosis) is a group of rare neurological disease. It involves the motor…
Q: Choose the correct statement/s regarding gene targeting in embryonic stem cells: gene targeting is…
A: Gene targeting is a replacement strategy based on the homologous recombination of the desired genes.…
Q: You run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker…
A: First of all, Western Blot is a technique which is used to detect the expression of protein from…
Q: What can you say about relative gene expression for the gene of interest, normalized to that of the…
A: The correct answer for the problem will be that the relative gene expression for the gene of…
Q: shown in the figure. Which of the following statements is true for the following experimental…
A: First, we need to know that what is the technique- q-rt-pcr? It stands for Quantitative Reverse…
Q: TOH1 protein is typically expressed at the same level in kidney and stomach cells and is essential…
A: Microarray is used to detect the expression of thousands of genes at the same time. DNA microarrays…
Q: The Hershey-Chase experiment, in which bacteria were infected with radioactively labeled bacterial…
A: The Hershey-Chase experiment, that also involved infecting bacteria with radioactively labelled…
Q: Match the stage of B-cell development in Column A with the rearrangement or expression status of the…
A: The B cells originate and mature in the bone marrow. They have the following stages while…
Q: A hereditary disease is inherited as an autosomal recessive trait. The wild-type allele of the…
A: Given Northern blot data:Wild type allele = 1250 nucleotides long and A 1020 nucleotide allele is…
Q: A revertant has been isolated from the E. coli mutant. The original mutant contains a mutation A136…
A: When the nucleotides sequences in the genome of an organism are altered or changed due to mistakes…
Q: In step 2 (above) in the identification of the colony that contains a targeted gene, ____. a.…
A: The shown steps are the part of colony hybridization to identify a gene of interest. Colony…
Q: Of the genes in the microarray shown in the lower part of Figure 20.10, are most overexpressed or…
A: Introduction: Microarray is a technique employed to study the expression of various genes. With the…
Q: You are handling a paternity lawsuit brought against five poten woman. You isolated DNA from the…
A: Answer. DNA fingerprinting DNA fingerprinting also known as DNA typing or DNA profiling is a…
Q: You have a mouse model that is a homozygous knockout mutant for the prnp gene (Prnp protein is not…
A: knockout mouse is a genetically engineered laboratory mouse (Mus musculus) in which a specific gene…
Q: ive the SIGNIFICANT difference between the terms provided in the table below.
A: Somatic Mutation Germline Mutation It occurs in the non-germline cells i.e. except…
Q: Cystic fibrosis (CF) is a genetic disorder affecting a number of organs, including the lung airways,…
A: many types of mutations are present which can explain the cause of the data we see above as cystic…
Q: Which of the following is an application of gene chips?
A:
Q: You are studying celiacs disease and have identified a potential gene for the disease using linkage…
A: Genetics is the study of the heredity, expression of genes and control of phenotypes. There are…
Q: . The website CBioPortal (http://www.cbioportal.org)is an exceptionally useful program for…
A: Oncogene is a gene that is responsible for causing cancer. Tumor-suppressor genes are normal genes…
Q: Please answer part 1A and 1B please I will upvote then with a proper explanation for the correct…
A: Micro satellites are short short DNA segments usually 1 to 6 base pairs that is repeated multiple…
Q: When doing the virtual DNA microarray analysis, which color (red, green or yellow) would the spot be…
A: DNA microarray analysis is a laboratory tool used to detect the expression levels of several genes…
Q: Three mutations were obtained in a bacterial gene. An antibody is available for the protein product…
A: "Genes" are the fundamental unit of heredity. They store genetic information in the form of DNA,…
Q: In cancerous cells, CpG islands are: where intercalating agents are found demethylated…
A: CpG stands for 5'—C—phosphate—G—3', which means that cytosine and guanine are separated by just one…
Q: In the Ames test shown in Figure 16-17, what is the reason for adding the liver extract to each…
A: Ames test is performed to detect the ability of a chemical to cause mutation in the DNA. Histidine…
Q: We are utilizing BL21 DE3 bacterial cells for the expression of the ADA protein via autoinduction.…
A: BL21 DE3 Bacterial cells: It is an E. coli B strain that lacks the Lon protease. Recombinant…
Q: Mutations in the ras gene family induce normal cells to proceed into the replication cycle. This…
A: Proto-oncogene normal function is to promote cell survival and cell division. Gain of function of…
Q: Cells that have deregulated expression of telomerase can become cancerous. This can be attributable…
A: Telomerase or terminal transferase is defined as an RNA-dependent DNA polymerase responsible for the…
Q: conditional mutation when there is an alteration to the coding region of a gene is caused by A.…
A: Changes in nucleotides in DNA may cause mutations. This my alter DNA sequence of a gene or make…
Q: A company that manufactures cleansing products has synthesized two new organic compounds , chemicals…
A: Disclaimer: Since you have posted a question with multiple subparts, we will solve the first three…
Q: Select from the options below the different types of mutations . Please select all that apply. -gene…
A:
Q: A. Which patient- 18, 30, or 1- shows the most microsatellite instability? Which patient shows the…
A: Microsatellite also called Simple Sequence Repeats (SSRs) or Short Tandem Repeats (STRs) are…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Pro's/con's of genetic testing. This can be as simple as for birth defects or in more complicated risk for different types of cancer.Breast Cancer Screening Program – A group of women were subjected to a breast cancer screening program. A newly developed screening test was utilized to measure its sensitivity and specificity against a gold standard test. a) What is the prevalence of breast cancer in this population? b) Calculate the Sensitivity of the test and provide interpretation c) Calculate the Specificity of the test and provide interpretationXeroderma pigmentosuma. is a genetic disorder that results in uncontrolled cell growth.b. is a genetic disorder in which the NER system is not fully functional.c. is a genetic disorder that results in the loss of pigment in certainpatches of skin.d. results from the lack of DNA polymerase proofreading.e. Both b and d are true of this disorder.
- what is the frequency of the rarest variant you can detect during deep sequencing of a tumor cell if you coverage is 250X? - 0.5% - 0.4% - 25% - 4%(a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.Briefly explain this Statement "Treatment for the genetic disorders by using gene therapy " Please answer at your own words, please (400-500 words).
- A genetic disease is caused by a deletion in part of a gene. The deletion results in one copy of the gene being shorter whereas the other copy is normal length. In the gel below, the DNA sequences of this gene from four individuals have been amplified using PCR (the gel is loaded at the top of the tray). Lane 1 of the gel shows an individual who does not have the disease. Which of the bands represents the disease allele? A B CWhich of the examples of genetic testing below are prognostic tests? Which are diagnostic? (a) Individual sequencing (personal genomics) identifies a mutation associated with Alzheimer’s disease. (b) ASO testing determines that an individual is a carrier for the mutant b@globin allele (bS) found in sickle-cell anemia. (c) DNA sequencing of a breast tumor reveals mutations in the BRCA1 gene. (d) Genetic testing in a healthy teenager identifies an SNP correlated with autism. (e) An adult diagnosed with Asperger syndrome (AS) has a genetic test that reveals a SNP in the GABRB3 gene that is significantly more common in people with AS than the general population.Answer each of the following questions correctly. a. How PCR may be used for the detection of disease–causing pathogens in a population during COVID Pandemic. For example: It may be used to check if a patient has a COVID virus infection. b. How cloning and expression of certain genes allows for massive production of the desired product. For example: the cloning and expression of insulin in bacteria allows for the mass production of this necessary protein for use by diabetic patients.
- What technique can be used for studying the gene for cystic fibrosis? a. cellular cloning b. molecular cloning c. reproductive cloningPlease answer all of these questions in details Answer all of the following questions a) Define the following terms: biotechnology, Plasmid,Genetic engineering and Transformation b) List two types of medical products that can be produced using DNA tech- nology c) Relate bioethics to the continued development of genetic engineering techniques. d) Describe two major goals of the Human Genome Project. e) Distinguish between proteomics and genomics. f) Outline the different applications of biotechnology g) List some techniques of genetic engineering. h) Delineate the transgenic organisms and their significance. i) Explain the procedure for the following PCR, Gel electrophpresis and Gene therapy j) Describe human genome project and its main goals. k) Discuss the drastic environmental impacts of oil spills and how the mi- crobes like bacteria can help mitigate the problem. l) Give an account of significance of transgenic organisms in food industry. m) Describe the uses of DNA finger…Which type of cells were used to extract the DNA that was sequenced? a. red blood cells c. white blood cells b. intestinal epithelium d. cheek swab What type of mutation caused Nicholas’s disease? a. frameshift c. nonsense b. missense d. insertion