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- a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total number of cells present in a patient’s specimen.b. After performing the streak plate method on a bacterial specimen, theculture was incubated for 48 hours at 37°C. Upon viewing the plate, therewas heavy growth (with no isolated colonies) in the fi rst quadrant, but nogrowth was apparent in the remaining quadrants. Please discuss errors in the procedure that could have produced this result.What staining method would you use to verify that the isolate is not producing survivalstructures against unfavourable conditions? Describe the procedure as wellWhy do DNA polymerases fromthermophilic microbes significantly improve the PCRprocedure?
- What are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank youWhy is the looping out method preferable for sputum specimen for acid fast smears?You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate method what other methods could you use to separate the bacteria? Please state what methods you would use, the result you would be looking for and interpret the result.
- Define triturate. Define supernatant. Why is SYBR green dye added to the gel? What is the centrifuge used for? What are restriction enzymes used for? What is a plasmid?How do you recognize individual colonies? Describe color, shape, size. Explain how the streak plate method enabled you to identify a single species from a mixed culture?Differentiate between agarose gel electrophoresis and SDS-PAGE based on composition of gels and dye used for staining.