Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: Give advantages and disadvantages of using commercially available kits for plasmid extraction over…
A: As molecular biology techniques get more sensitive, a DNA extraction kit that yields high-quality…
Q: how do you purify a protein for single particle cryo-electron microscopy?
A: We would require a protein sample with more than 99% purity in order for it to qualify as a sample…
Q: Define fluorescent staining.
A: The advent of microscopy started with the development of optical microscopy. Then the level of…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: What is gel electrophoresis and why is it important? Write in detail.
A: Electrophoresis is the technique that is used to separate biomolecules that have a charge in them.…
Q: Create a flow chart on how gel electrophoresis is being conducted
A: Gel electrophoresis is a molecular technique that helps us to separate a mixture of DNA sample based…
Q: Transillumination is used clinically for the detection of:
A: Transillumination is an examination that is done with the help of a strong light shining on a…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: What are the principle and basic concepts of DIFFERENTIAL staining?
A: The simple dye is used in the technique of simple staining to highlight only the specific structures…
Q: What are the differences among native gel electrophoresis, SDS-PAGE, isoelectric focusing,…
A: The separation techniques are essential to identify and isolate biomolecules for further biological…
Q: Define gel electrophoresis?
A: DNA is vital for all living beings. It is important for inheritance, coding for proteins, and the…
Q: In four steps, describe the simple staining procedure.
A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…
Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…
Q: describe the agar-overlay technique
A: Biological techniques are processes or methods for trying to study living things. They include…
Q: Why is it important to use a sterilized loop between streaks when preparinga streakplate?
A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: Å Q/ Why don't we fixation to simple negative staining?
A: It is method followed to help view a specimen under the microscope to study its morphological…
Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…
A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…
Q: Amazingly, the sensitivity of STR profiling requires only ___________ DNA-bearing cells to obtain an…
A: Deoxyribonucleic acid or DNA is the hereditary material that is present in all organisms. DNA…
Q: Define SDS gel electrophoresis.
A: Electrophoresis is the process of a charged particle migrating under the influence of an electric…
Q: What side of the electrophoresis chamber (red or black) should the wells be on when the gel is…
A: The agarose gel electrophoresis is a technique that is used to separate DNA fragments as per their…
Q: What is the difference between SDS-PAGE and 2D electrophoresis
A: SDS-PAGE AND 2D electrophoresis- Both are isoelectric focusing mean also known simply as electro…
Q: Draw and explain the process of electrophoresis utilized in decalcification.
A: Decalcification :- As name suggests, it is removal of calcium salts,minerals and calcium tissues…
Q: When working with the gel electrophoresis chamber, what must we keep in mind?
A: "Gel-electrophoresis" is a widely used technique for separating the fragments of DNA(or other…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: When run under nondenaturing conditions (without SDS or β-mercaptoethanol), native polyacrylamide…
A: Gel electrophoresis is the technique of separation of molecules on the basis of their size and…
Q: Write how can prepare 5% gel for gel electrophoresis.
A: We are answering for 0.5% gel for gel electrophoresis, as common range of gel is from 0.5% to 2% for…
Q: write the protocol for mann's methyl blue-eosin staining, including their reagents, materials,…
A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…
Q: Explain the function of stacking gel, separating gel, and Coomassie blue stain in the…
A: Answer : Stacking Gel : The purpose of the stacking gel packing is to combine all the samples of the…
Q: Differentiate the “hot method” and “cold method” of acid-fast staining
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Name the substance used as a medium in gel electrophoresis.
A: Electrophoresis is the process of separation of charged macromolecules under the influence of…
Q: Besides agarosc gel electrophoresis, there is also macromolccules. Differentiate both these methods.…
A: Agarose gel electrophoresis and SDS-PAGE are techniques for separation of macromolecules.
Q: list the stains and decolorizing agent used in acid fast staining method.
A: Certain bacteria have a physical property known as acid fastness. Property:- Resistance to…
Q: List four reasons for the development of pulsed field gel electrophoresis
A: Electrophoresis is a general term that describes the migration and separation of charged particles…
Q: Nucleic Acid Hybridization Technique Northern Blot Western Blot Transblot Molecules involved (DNA,…
A: Nucleic acid hybridization technique Northern blot Western blot Transblot…
Q: List the step-by-step procedure of Gram staining.
A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…
Q: Explain the conclussion about gel electrophoresis using 3 different food dyes (yellow, blue and…
A: The method used to separate mixtures of DNA, RNA, or proteins according to their size is known as…
Q: Define dyes and describe the basic chemistry behind the processof staining.
A: Detection and observation of cells and microorganisms in their natural state under microscope is…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…
Q: What is the use of a “ladder” on a gel electrophoresis?
A: When there is a mixture of nucleic acids and proteins, they can be separated according to their…
Q: ative gel electrophoresis
A: Separation techniques are critical for identifying and isolating biomolecules for further biological…
Q: Discuss the functions of the following components in performing gel electrophoresis;…
A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…
Q: Explain the methods and materials in gel electrophoresis using 3 different food dyes (yellow, blue…
A: Gel electrophoresis is an analytical technique used in the lab to separate mixtures of DNA, RNA…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: Outline the techniques of two-dimensional gel electrophoresisand tandem mass spectroscopy, and…
A: Two-dimensional gel electrophoresis is the primary technique for proteomics work. It separates the…
Q: Explain situations in which pulsed-field gel electrophoresis is most useful.
A: Numerous bacteria cannot be cultivated using the same genotypic techniques, according to genetic…
Step by step
Solved in 2 steps
- Briefly discuss the difference between simple and differential staining? Which is better suited for common clinical microbiology laboratory applications?What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.Explain the methods and materials in gel electrophoresis using 3 different food dyes (yellow, blue and purple)
- Please briefly explain what gel electrophoresis is and how it works to separate a mixed sample of macromolecules like DNA.What can be done with the food dye to utilize it as an additive biocompatible material to improve the multilevel complex 3D bioprinting process? What is the process? What technology is involved?list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet endospore staining methods. (omit the rinsing steps)