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- A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
- Describe (at the cellular level) how detergents like ostrasan and SDS (from solution 2 in the plasmid extraction) sterilize surfaces. (Answer for each detergent, give info about what it does at the cellular/molecular level.) A. Ostrasan B. SDS State your sources.POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What is your observation from potato A to D? 2. What can you conclude by comparing potato A and potato B? 3. What can you conclude by comparing potato A and potato C?POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What can you conclude by comparing potato A and potato D? 2. The liquid in the cavity of some potatoes came from where? 3. What is your conclusion about the experiment?
- E. coli can grow at a higher temperature in a complex mediumthan in a defined medium. Why?Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.mixed micron cuboidal shaped cells within the stronger paste (F127) and the physical/biochemical process that occurs during the gelation
- Explain the advantages and disadvantages of Differential Centrifugation, main differences btw. Differential and Gradient Density Centrifugation and why the differential Centrifugation is a process of successive centrifugation?How to write the methodology? I have a practical of centrifugation, I need to do a lab report, but I don't know how to write the methodology. Here is the introduction: The centrifuge is a common and widely used instrument in the laboratory. In biology,centrifugation is a technique used to separate biological particles or macromoleculesfor instance cell debris, sub-cellular components, proteins, nucleic acids, viralvaccines, antibodies and etc. Principally, the separation is based on the particle size,shape, and density where large and high-density particles travel at a faster rate andwill be sedimented first, followed by other particles. There are many different types ofcentrifuges that are commercially available such as large-capacity low-speedpreparative centrifuges, refrigerated high-speed preparative centrifuges, analyticalultracentrifuges, preparative ultracentrifuges, large-scale clinical centrifuges, andsmall-scale laboratory microfuges.You have a suspension of cells at a concentration of 5 x 106 cells/mL in culture media. The protocol you’re using for your experiment asks you to dilute the cells 1:10 in fresh culture medium to make a 20 mL suspension. How much of your original suspension and fresh culture media will you need? What’s the concentration of cells now in your working suspension?