QUESTION 22MstdigestonHb*I Hb HDI HD Hb*I HbE 1.35 kbE 1.16 kbSouthern blotThe figure above is a diagram of a Southern blot using a probe for the hemoglobin Hb gene. The blot shows a restriction fragment polymorphism (RFLP)related to the sickling trait (Hbs) in an Mst II restriction enzyme digest. Whích of the following statements about the location of the probe iscorrect?O A The Hbs mutation destroys an Mst II restriction site located within the probe hybridization region.O B. The Hbs mutation creates a new Mst II restriction site located within the probe hybridization region.O C. The probe hybridization region is completely contained on a 0.2 kb MstII restriction fragment.O D. The probe hybridization region is completely contained on a 1.15 kb MstII restriction fragment.O E. None of the above is correct.

As indicated in the RFLP and restriction digestion analysis of the HbA and HbS gene there is a…

Answered: QUESTION 22 Mst digeston Hb*I Hb HDI HD…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 1. Although we will not be doing a gel electrophoresis, data from a gel digest of a Bacillus anthrax plasmid is provided so you can do a DNA map. The Bacillus anthrax plasmid is 4000bp (4Kb) long. Note the origin position as well as the reference molecular weight markers on the gel. Two restriction enzymes, A and B, were used to obtain two individual digests, A and B. They were combined to produce the third digest. The restriction enzyme fragment pattern for the digest of Bacillus anthrax plasmid Determining the Number of Fragments How many fragments were produced by enzyme A? How many fragments were produced by enzyme B? How many fragments were produced by the combined digest (A and B)? Fragment Size Fragment size is relative to molecular weight, and must be determined by comparing the fragment distance to the molecular weight markers. The fragment size has been provided on the gel pattern for this exercise. To make a map you must determine the relative positions of the…
QUESTION NO. 1 A transition mutation A. occurs when a purine is substituted for a pyrimidine or vice versa. B. results from the insertion of one or two bases into the DNA chain. C. is most frequently caused by chemicals (like acridine) that intercalate into DNA. D. results from substitution of one purine for another or of one pyrimidine for another. E. always is a missense mutation QUESTION NO. 2 Degeneracy of the generic code denotes the existence of A. multiple codons for a single amino acid. B. codons consisting of only two bases. C. base triplets that do not code for any amino acid. D. different systems in which a given trip let codes for different amino acids. E. codons that include one or more of the unusual bases. QUESTION NO. 3 Replication A. requires that a phosphodiester bond of the incoming dNTP be hydrolyzed in order to be added to the growing chain. B. uses 5' to 3' polymerase activity to synthesize one…
QUESTION 10 Remdesivir is converted in vivo to CMP analog and then phosphorylated to CTP analog. a successful treatment for Ebola. There are more than 1 correct answers in the other choices. a potential chain terminator for the replication of RNA viral genome
QUESTION 24 During lagging strand synthesis of DNA, Okazaki fragments are linked together by ___________. DNA polymerase I Primase Beta clamps DNA Ligase
QUESTION NO. 1 Fragile X syndrome is a common form of inherited mental retardation. The mutation in the disease allows the increase of a CGG repeat in a particular gene from a normal of about 30 repeats to 200-1000 repeats. This repeat is normally found in the 5' untranslated region of a gene for the protein FMR1. FMR1 might be involved in the translation of brain-specific mRNAs during brain development. The consequence of the very large number of CGG repeats in the DNA is extensive methylation of the entire promoter region of the FMR1 gene. Methylation of bases in DNA usually A. facilitates the binding of transcription factors to the DNA. B. makes a difference in activity only if it occurs in an enhancer region. C. prevents chromatin from unwinding. D. inactivates DNA for transcription. E. results in increased production of the produce of whatever gene is methylated.QUESTION NO. 2 The best definition of an endonuclease is that it hydrolyzes A. nucleotide from…
QUESTION 27 Which of the following methods can be used to compare the amounts of one specific mRNA that is expressed by two different cell lines? A. Immunohistochemistry B. Western blotting C. Polymerase chain reaction (PCR) D. Immunocytochemistry
QUESTION 5You are asked to recommend an antibiotic to treat potential pathogenicityt in an animal. Describe a method which will allow you to make this recommendation.
QUESTION 25 During lagging strand synthesis of DNA, the RNA primers are replaced with DNA by __________. DNA Polymerase I DNA Polymerase II DNA Polymerase III Primase
Question 6 In the first part of the experiment, there were only a few colonies on the streptomycin positive plate. In the second part, there are a large number of colonies on the streptomycin positive plate that was inoculated with the antibiotic resistant strain. What is the most likely explanation for this difference? 1. The antibiotic resistant strain was composed of a large number of individuals with resistance to streptomycin 2. The exposure to streptomycin caused a mutation in a few individuals
QUESTION 24 Human immunodeficiency virus (HIV) is of interest to evolutionary biologists because all of the following, EXCEPT: a. Phylogenetics allow us trace epidemics and transmission pathways. b. Evolution of the virus has led to drug resistance and the need for multidrug treatments. c. Population demography and disease transmission can differ between populations. d. Resistant alleles are maintained in high frequency due to high selective pressure of the disease.
The genetic code is identical in cyanobacteria, lions, and humans Question 25 options: True False
Question 23 Heterochromatin is the part of the DNA where genes are transcribed actively Question 23 options: True False Question 24 Chromatin Immunoprecipitation is used to : Question 24 options: Isolate DNA binding proteins Identify specific sequences bound to Transcription factors Identify a DNA binding peptide Identify high throughput sequence

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