Bradford Assay is most suitable to use when the extraction buffer is below the target protein’s pI. This is so because the protein would be more a. Positively charged allowing the CBB G-250 dye to bind via its sulfonate groups. b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate groups. c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate groups. d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.
Bradford Assay is most suitable to use when the extraction buffer is below the target protein’s pI. This is so because the protein would be more a. Positively charged allowing the CBB G-250 dye to bind via its sulfonate groups. b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate groups. c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate groups. d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Bradford Assay is most suitable to use when the extraction buffer is below the
target protein’s pI. This is so because the protein would be more
a. Positively charged allowing the CBB G-250 dye to bind via its sulfonate
groups.
b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate
groups.
c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate
groups.
d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.
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