Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). Youmonitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing clactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growingexponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucosemedia is 20 minutes. You did not change the oxygen content or temperature at Which you were growing the cells, E. coli is still aerobicallyrespiring at 37°C.Question: If the lac operon is 'on' what does this imply about the cell?O The cell is dying and the lac operon is going to induce apoptosis. Apoptosis is truly cell death and the cells will enter into the death phase of the GrowthCurve.O Lactose is being catabolized and glucose is no longer present. In addition, the inducer allolactose, is inhibiting the repressor protein.O Glucose is being catabolized by the cell and it will efflux lactose. In addition, CAMP levels are high and inhibiting the promoter.O Genes for lactose catabolism are being transcribed as the repressor allolactose is no longer blocking the operator region of the operon.No new data to save. Last checked at 8:18pmSubmitMacBook Air

The prokaryotic gene expression is regulated by the operon system. By this operon system many…

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growin lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growir exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobica respiring at 37°C. Question: If the lac operon is 'on' what does this imply about the cell? O The cell is dying and the lac operon is going to induce apoptosis. Apoptosis is truly cell death and the cells will enter into the death phase of the Growth Curve. O Lactose is being catabolized and glucose is no longer present. In addition, the inducer allolactose, is inhibiting the repressor protein. O Glucose is being catabolized by the cell and it will efflux lactose. In addition, CAMP levels are high and inhibiting the promoter. Genes for lactose catabolism are being transcribed as the repressor allolactose is no longer blocking the operator region of the operon.

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter19: Biomedical Laboratory Instrumentation

Section: Chapter Questions

Problem 2RT