After incubation of the mating mixture for 1 hour, prepare a serial dilution of the mating mixture in sterile saline (to 10-3). As described in step 2, clearly label each of the double selective agar plates (i.e. (i) Nutrient agar+ Ap + Rif, (ii) Nutrient agar+ Sm + Rif) AND iii) the Nutrient agar + Ap + Sm + Rif plate. Spot inoculate 3 x 10 µl drops of the undiluted, 10-1, 10-2 and 10-3 dilutions of the mating mix onto quadrants of each of these plates. • What is the purpose of this step?
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- 1. how does colony appearance and location with respect to the agar differ on the spread and pour plate? 2. list two additional factors that contribute to error in the spread plate method:6. After culturing organisms on mannitol salt agar overnight at 37 degrees, one of them grew on MSA but did not generate a yellowing of the media. Which of the following could be interpreted from the results for this organism? A. Salt-tolerant Staphylococcus that does ferment mannitol. B. Organism is inhibited by salt and does not ferment mannitol. C. Salt-tolerant Staphylococcus that does not ferment mannitol. D. Organism is inhibited by salt and does ferment mannitol.1. List the different reagent strip tests with their principle 2. Enumerate 5 clinical significance for each reagent strip tests
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- 1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.1. How would you interpret the following result of the Bile Esculin Test: "Growth of the bacteria is present however no darkening of the agar can be observed after 48 hours." Please explain.(2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specific
- 1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses. 2.volume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h. (answer:P = 67,55 g/L)1. You were given a mixed nutrient agar broth culture of bacteria. a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?