Define aseptic technique and briefly explain what we should do in the lab to provide aseptic conditions?

Q: What are the advantages and disadvantages of using fi ltration as a method of control?

A: Filtration is the separating of substances based on their different chemical and physical qualities.…

Q: Describe the purpose of microbial testing?

A: Microorganisms come in contact with us everyday in which some are harmless and some can cause severe…

Q: Describe the mechanism of SERS( Surface Enhanced Raman Spectroscopy).

A: Introduction: Surface-enhanced Raman spectroscopy (SERS) is a strong microscopic observation…

Q: What are the pros and cons of serial dilution method and W/V % method?

A:

Q: How are the Kato Katz Technique results reported?

A: The Kato–Katz technique is a laboratory procedure for preparing human stool samples  parasite egg…

Q: Discuss three physical states of media and when each is useful.

A: Culture media, also termed growth media, is defined as the specific mixture of nutrients and other…

Q: What is the difference between culture-based technique and culture-independent techniques based on…

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: explain how beta and gamma radiation can be used to produce diagnostic images.

A: Beta and gamma radiations are both ionizing radiation that can pass through the human body. They are…

Q: Would phase microscopy be of any particular value when examining a stained specimen? Explain

A: The area or field of study in which microscopes are used to view an object that cannot be seen…

Q: To establish a standard curve for a BSA standard curve using Bradford, the spectrophotometer…

A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…

Q: What are the purposes of "BAG TECHNIQUE PROCEDURE" and why is it important that you should properly…

A: Community bag is a type of tool that is used for home visit and enable community health nurse to…

Q: explain why it is important to maintain sterility when inserting a foley cathether

A: Foley's catheter is an indwelling catheter, i.e. it can be left in place for longer durations than…

Q: Discuss three physical states of media and when each is used

A: The food substance or substances required for growing micro-organisms in vitro is called the culture…

Q: What specific medium must be used in testing the effectiveness of antimicrobial drugs?

A: The specific medium that must be used in testing the effectiveness of antimicrobial drugs is…

Q: Calculate the size of an organism that occupies 26 um spaces under the scanner, LPO, and HPO using…

A: An ocular micrometer is used to measure the size of objects. The stage micrometer is used to…

Q: Differentiate between bright-phase and dark-phase microscopy in terms of phase shift.

A: INTRODUTION:- brightfield,darkfield and phase contrast are 3 contrast enhancement techniques in…

Q: Describe in detail the process of electrospray ionization ?

A: Ionisation is done by high voltage. Ions spread evenly on a surface. Ionised liquid gives rise to…

Q: Describe procedure of RFLP.

A: RFLP (restriction fragment length polymorphism) is an inherited difference in the pattern of…

Q: Explain the usage of photoactivated localization microscopy technique.

A: Photoactivated localization microscopy abbreviated as PALM is a microscopy technique that was…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: Differentiate the Bright-field and a Dark-field microscopy.

A: Microscopy -- The word microscopy is derived from the Greek word  micros - small Skopeo - to view…

Q: describe in detail how to perform a qualitative analysis and a quantitative analysis using…

A: INTRODUCTION   Paracetamol, N (4hydroxy phenyl acetamideis analgesic and antipyretic drug.It is used…

Q: Provide three reasons why the use of aseptic technique is essential when handling microbial cultures…

A: Aseptic techniques means using practices and procedures to prevent contamination from pathogens. It…

Q: Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.

A: Proteins are defined as the biological molecule that are produced by the building blocks amino…

Q: What is selective medium?

A: Description of a selective medium.

Q: What is there to understand about Aseptic Transfer for Aseptic Technique

A: Microbes are present everywhere. They are present in the air, they can be sticking on your hands,…

Q: Explain the use of mass spectrometry (MS) ?

A: Mass spectroscopy is a laboratory analytical tool. It is used to detect the mass-to-charge ratio of…

Q: Discuss the history and development of aseptic techniques.

A: Aseptic strategies were first generally embraced in the late nineteenth century. Preceding this, the…

Q: Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization…

A: Technology is utilized in science, while science is used in technology. Both are vital to our…

Q: When it comes to the sensitivity of the sensors, which one will be more preferred asa biosensor, the…

A: Biosensors are the devices that are used to detect bioanalyte elements such as biomolecule…

Q: Principle of Neutralization Tests (NT).

A: In a neutralization test, serum and virus are reacted together in equal volumes and inoculated into…

Q: What is PET (positron emission tomography) scans can?

A: Positron Emission Tomography (PET), which employs injections of radiopharmaceuticals to help a…

Q: Differentiate between microscopic and macroscopic methods ofobserving microorganisms, citing a…

A: Microscopes are inevitable in the study of microorganisms. They allow for microscopic observation of…

Q: How do you maintain an aseptic environment in the laboratory?

A: Microorganisms are useful in various fields that include the food industry, biofuels, wastewater…

Q: What is the main principle that governs aseptic technique? Explain in minimum of 4-5 sentences.

A: Answer

Q: What are the key considerations for the scale-up of cell culture process for the large molecule drug…

A: Well developed skills are needed to increase the batch yield and to reduce processing time for large…

Q: What could be possible errors when you incorrectly identify an Unknown organism when using IMViC…

A: IMViC tests are used to identify bacteria from specimens collected from patients in majority of…

Q: Which Two types of electron microscopy are in routine use in microbiology? Explain

A: The electron microscope is an instrument that uses the beam of electrons to examine objects on a…

Q: In 5-8 sentences, explain the importance of the Aseptic technique in PERFORMING LABORATORY…

A: It is important because it keeps infectious microorganisms from sterile surfaces or tissues. It…

Q: Give a vivid outline of the Standard operating procedure of neutralization test.

A: The neutralization test is a diagnostic test helps to measure the patient's ability to protect cell…

Q: What are the significance of UV/Vis spectrophotometer in the experiment complexation.

A: UV/VIS spectrophotometer is the technique used to determine the optical properties  such as…

Q: Briefly explain the technique for preparation and interpretation of disc diffusion tests.

A: Antimicrobial drugs are natural or manmade chemical compounds that inhibit or eliminate the growth…

Q: What are the principles of pulse oximetry technology?

A: ▪︎Pulse oximetry is a simple, relatively cheap and non-invasive technique to monitor oxygenation.…

Q: Describe three ways to increase the depth of penetration of electrical impulses when using pad…

A: Automated external defibrillators are portable electronic devices that automatically diagnose…

Q: Explain situations in which pulsed-field gel electrophoresis is most useful.

A: Numerous bacteria cannot be cultivated using the same genotypic techniques, according to genetic…

Q: What is the principle of freezing of cell lines and primary cells. Describe the general protocol for…

A: Cryopreservation  It is defined as a process of preserving cells, tissues, organs, or any type of…