What is the principle of freezing of cell lines and primary cells. Describe the general protocol for freezing cultured cells.

Q: What is the principle of freezing of cell lines and primary cells.Describe the general protocol for…

A: Cell culture is a procedure in which cells (either animal or plant cells) are separated from an…

Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…

A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…

Q: Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer

A: Disclaimer: “Since you have asked multiple questions, we will solve the first question for you. If…

Q: Why is dilution a necessary part of pure culture preparation?

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: How does pasteurization affect the number of bacteria present in milk? What differences, if any,…

A: To control microbial growth or contamination there are a number of aseptic techniques developed that…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Why are biochemical tests used to identify microbes?

A: Microbes are living organisms that are very small and can be seen only with the help of a…

Q: Explain how are the physiological characteristics of bacteria identified using biochemical test?

A: The physiological properties that are used for the identification of bacteria includes the nutrient…

Q: Why does a streaking method used to inoculate plates result in isolated colonies?

A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…

Q: to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is…

A: Motility is the ability of an organism to move from one place to other, generally, the bacteria…

Q: How to do cellulolytic characterization using a 96-well plate? What is the protocol or procedure to…

A: Cellulolytic action can be measured by an assortment of techniques that have been summed up in…

Q: What is a pure culture?

A: Introduction  Culture is referred to th process of growing the cell outside the animal or plant body…

Q: What order should the steps be in for this culture method?

A: A conditional mutant is a mutant that exhibits a wild-type phenotype under permissive conditions but…

Q: Which of the following cannot be grown in artificial media and must be grown in live animals or…

A: Artificial media is a medium that is defined. This medium is chemically defined. It is a synthetic…

Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…

A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…

Q: How can the enrichment culture technique be used medically?

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: Why must primary cell cultures be restarted every so often when preparing primary cell cultures to…

A: Primary cell cultures are derived from tissue and they are more similar to the in vivo state and…

Q: Under what conditions are media typically sterilized in an autoclave?

A: An autoclave is the pressure chamber used to carry out the scientific and industrial processes…

Q: How does gel filtration work? What are the applications of analytical versus preparative versions…

A: Chromatography is a technique for separating a mixture in the laboratory. The mixture is dissolved…

Q: What is the procedure of beta method?

A: Beta-lactamase test is used for the detection of enzyme beta-lactamase. This enzyme provides the…

Q: Biochemical and differential tests can be incorporated into growth media or performed on isolated…

A: It is required to provide three examples of differential test that is incorporated into growth media…

Q: what are the important principle in invertase test?

A: To determine enzymatic activity, the amount of substrate remaining or the amount of product created…

Q: What is immunofluorescence microscopy

A: Immunofluorescence microscopy is a technique used for light microscope with a fluorescent microscope…

Q: Why must materials and instruments used in tissue culture be kept sterile?

A: Sterilization of explants is a key step in plant tissue culture.

Q: What advantages do solid media offer for the isolation of microorganisms?

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: What is the most important instrument in an animal cell culture laboratory?

A: The process by which animal, insect or human cells are isolated from a living tissue and grown in…

Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Using a schematic diagram, summarize the following steps in preparing competent cells for…

A: prepare competent cells for transformation ;- Add 20 µL of each ligation reaction or 1 ng of uncut…

Q: If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: What are the advantages and disadvantages of using the Wet Mount technique?

A: As per bartleby guidelines, we are suppose to answer only one question kindly post other separately.…

Q: Name the substance used as a medium in gel electrophoresis.

A: Electrophoresis is the process of separation of charged macromolecules under the influence of…

Q: Why differential centrifugation can cause contamination in pellet?

A: Differential centrifugation is a typical process used in microbiology and cytology to separate…

Q: What is the most commonly used fixative in stool samples and why?

A: Fixative is a moist environment for transporting and preserving intestinal parasites in stool…

Q: What is the advantage of cryo-sectioning for antibody staining methods?

A: To study the antigenic structures, present on the cell surface tissues can be embedded in paraffin…

Q: What is the procedure for methylene blue stain on e.coli?

A: Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as…

Q: Outline in detail the procedure for celloidin embedding .

A: Embedding is that the method during which the tissues or the specimens are capsulate in a very mass…

Q: What are the advantages and disadvantages of using the Slide Culture technique?

A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…

Q: How do we isolate culture media (step by step process) and what is the purpose of bacterial culture…

A: Introduction :- Microorganisms can be found in the natural environment, such as soil. They coexist…

Q: what is the procedure of solving the probelems of cultures those are suffering severely due to…

A: This is the defense compound of the plant and are toxic in nature because of that preventing plant…

Q: E. coli O157:H7 is an organism of concern in contaminated foods. How can the use of MacConkey agar…

A: MacConkeys Agar is a selective and differential culture medium. It specifically select Gram negative…

Q: What is the structural and functional differences between Inoculating loop versus inoculating…

A: Inoculating tools  These tools are use by microbiologist to inoculate microorganisms easier.

Q: Define the following terms; Sterilization, and Aseptic Technique. What are the differences between…

A: Microbes invading and proliferating within an organism's body causes infection. Microbes include…

Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…

A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…

Q: What are the possible contamination in animal cell tissue culture laboratory and list important…

A: Animal cell culture is a technique in which the cells are grown obtained from animals are grown…

Q: Briefly, in your own words, describe the steps of the gram staining procedure.

A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…

Q: What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of…

A: Microbes are all pervading. They are found everywhere, in air, water, any substratum, inside and on…

Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?

A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…