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- Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425 Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425a. Calculate the retention factor for each soluteb. Calculate the selectivity factorc. How many plates are needed to provide a resolution of 1.5?d. What column length should be used to provide a resolution of 1.5 if the plate height ofthe packing is 1.5 ×10-3 cm?e. If the flow rate is 6.75 cm min-1, how long will it take to elute the two species?A sample of steel (0.506 g) was digested in acid and the solution made up to 100 cm3 with deionised water. Aliquots (25.0 cm3) of this solution were transferred into two volumetric flasks (100 cm3) labelled A and B. Flask A was made up to volume with deionised water. To flask B was added 10.0 cm3 of a 260 ppm standard solution of Mn2+ which was then also made up to volume with deionised water. The following absorbance results were obtained: Solution A = 0.356, Solution B = 0.578. Use these results to calculate the percentage (% w/w) of manganese in the steel sample.The molar absorptivity of Allura Red is 2.59 x 104 M-1 cm-1, account for the accuracy of the modified set-up. What could be the factors that contribute to the inaccuracy or the modified spectrophotometric set-up?
- The following volumes of a solution containing 1.10 ppm Zn2+ were pipetted into separatory funnels each containing 5.00 mL of an unknown zinc solution: 0.00, 1.00, 4.00, 8.00 and 12.00. Each was extracted with three 5 mL aliquots of CCl4 containing an excess of 8-hydroxyquinoline. The extracts were then diluted to 25.0 mL and their fluorescence measured with a fluorometer. The results for the standard addition calibration were as follows: Volume Std. Zn2+ / mL [Zn2+] / ppm Fluorometer Reading 0.00 6.12 1.00 7.41 4.00 11.16 8.00 15.68 12 20.64 a) Determine the concentration on Zn2+ ions in each of the standard solutions coming from the coming from the 1.10 ppm Zn2+ solutions, Please show your working1. Discuss briefly the specifications and test parameters for system suitability for HPLC analysis.In the food dye experiment, the molar absorptivity of Allura Red is 2.59 x 104 M-1cm-1, account for factors that may affect the accuracy of the modified spectrophotometric set up?
- A sample of body serum is to be analysed for sodium by flame emission spectroscopy. 1.00cm' aliquot of serum was pipetted into each of two 50.0cm' volumetric flasks. The first flask was diluted to volume with deionised water. The absorbance of this first solution was0.350. To the second flask 10.0 cm' of a 25.0 ppm sodium standard was added and the flask made up to volume with deionised water. The absorbance of this second solution was 0.720.(i) Calculate the concentration of the sodium in the body serum in mg dm-3.Using the absorbance of [FeSCN]+2 at the wavelength of maximum absorption which you obtained in part B, and the diameter of the tube, obtained in part A,calculate the molar extinction coefficient. given info: Concentration of Fe3+ stock solution, C1 = 0.001 M Volume of stock Fe3+ solution pipetted, V1 = 25mL Concentration of diluted stock solution, C2 = 0.0004 M Volume of Fe2+ solution and KSCN (upon mixing) = 10mL Weight of KSCN = 1g The reaction taking place is given as: The reaction taking place is given as: F e 3+ (aq) + SC N − (aq) ⇔Fe [SCN] 2+ (aq) the concentration of [FeSCN]2+ after the mixing of Fe3+ and SCN- is 2.5 x 10-3 M. Moles of [FeSCN]2+ = 2.5 x 10-5 moles Diameter:1.2cm The wavelength at which maximum absorption takes place is = 480 nmTwo species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425a) What column length should be used to provide a resolution of 1.5 if the plate height ofthe packing is 1.5 ×10-3 cm? b) If the flow rate is 6.75 cm min-1, how long will it take to elute the two species?
- Calculate the amount of phycocyanin in Sample 1 in mg where A620 = 0.193 and A650 = 0.095, taking into account the dilution factor of 100 ul, and the total volume of extract 45ml. Note your answer to 2 decimal places.state the three different modes of GC/MS, and explain more for each.. If the enthalpy change of an enzymatic reaction associated with the conversion of 2000 g of a target analyte substrate to 1 mol of the product is -100 kj/mol, the heat capacity of the system is 1 kj/K.Kg, Seebeck coefficient is 0.01 V/K, then estimate the output potential difference (ΔV) of a thermopile, with one pair number of the thermocouple.
