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state the three different modes of GC/MS, and explain more for each.
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Solved in 2 steps
- Why is GSC not used nearly as extensively as GLC?Give the rationale behind major steps in the analysis such as blending, filtration, heating of the sample or standard with DNS, dilution, and absorbance measurement.With the aid of a well labelled block diagram, briefly describe the functions of the major components of gas chromatography (GC). What is the difference between the samples separated by GC and HPLC?
- 1. What is the significance of Rf values?2. What is the basis for the separation of components of mixtures by paperchromatography?3. Give 3 important applications of chromatography.4. Define or describe each of the following terms: - Chromatography- Development- Solvent front- Stationary phase - Chromatogram- Adsorbent- Mobile phase- RfDifferent from GCMS, why is an interface needed when coupling HPLC with MS?A spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.
- Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the thin layer chromatography of plant pigments? How do you say so?In reversed phase HPLC, how will the retention time of a component of a mixture vary with the polarity of its molecules? Explain your answerDifferentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the video demonstration in TLC and paper chromatography? How do you say so?
- Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the demonstration in TLC and paper chromatography? How do you say so?(h) Describe a chromatographic technique that could be suitable for studying the molecular weight distribution of poly(phenylenevinylene) derivatives that are soluble in organic solvents. Explain the physical basis on which separation could occur and suggest how the polymers could be detected after elution.I don't understand how you calculated the w1/2 for part c... where did the 5.54 come from? And can you answer the last part of the question-> d) If the volume ratio of the stationary phase to mobile phase is 0.25, find the distribution constant for compound 1.