describe the process of transcription of DNA based off of these figure If the RNA polymerase followed the template strand around the axis of the DNA duplex,there would be no strain, and no supercoiling of the DNA would occur, but the RNA chainwould be wrapped around the double helix once every 10 base pairs. This possibility seemsunlikely because it would be difficult to disentangle the transcript from the DNA complex.RNARNApolymeraseТорoisomeraseremoving negativesupercoilTopoisomeraseremoving positivesupercoilRNApolymeraseAlternatively, topoisomerases coukd remove the supercoils. A topoisomerase capable of relaxingpositive supercoils situated ahead of the advancing transcription bubble would "relax" the DNA.A second topoisomerase behind the bubble would remove the negative supercoils. RNApolStep 1 Recognition of promoterby o; binding of polymeraseholoenzyme to DNÁ;migration to promoter5'Promoter3'5'DNA templateStep 2 Formation of an RNA5'polymerase:closed promoter 3tcomplexStep 3 Unwinding of DNA atpromoter and formation3of open promoter complexPurine NTPStep 4 RNA polymerase initiatesmRNA synthesis, almostalways with a purine5'3'5'PPP FaNTPSStep 5 RNA polymerase holoenzyme- 3'[catalyzed elongation ofMRNA by about four morenucleotides3'PPP PuNINININAStep 6 Release of o-subunit as coreRNA polymerase proceedsdown the template, elongatingRNA transcriptPromoter3'P P P Pu NNNNI5'in ioo ioe io

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Description: describe the process of transcription of DNA based off of these figure If the RNA polymerase followed the template strand around the axis of the DNA duplex,there would be no strain, and no supercoiling of the DNA would occur, but the RNA chainwould b

Transcription is the process of the formation of RNA copy from the DNA. The main enzyme responsible…

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Biology

describe the process of transcription of DNA based off of these figure

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter29: Transcription And The Regulation Of Gene Expression

Section: Chapter Questions

Problem 3P: Substrate Binding by RNA Polymerase RNA polymerase has t binding sites for ribonucleoside...

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52Protein synthesis takes place on ( ). 53The eukaryotic pathway of polypeptide chain elongation is the same as the bacterial pathway except that eEF1A and eEF2 replace eF1A and EF2. Yesorno 54Which experiment show that σ70-RNA polymerase combines with promoter DNA to form a closed and an open complex?A.Footprintingb.Southern blottingc.ComplementationD.DNase protection
Some events that may occus during transcription 1. RNA polymerases detach 2. DNA polymerases detach 3. DNA polymerases proofread 4. RNA double helix is unzipped 5. DNA double helix is unzipped 6. DNA double helix forms again 7. Free nucleotides in the nucleus bond to exposed nucleotides of the senes strand 8. Free nucleotides in the nucleas bond to eposed nucleotides of the sense antisense strand 9. RNA ligase fuses the Okazaki fragments 10. RNA polmerase binds to the sense strand 11. DNA polymerase binds to the sense strand 12. RNA polymerase binds to the antisense strand 13. DNA polymerase binds to the antisenes strand List in sequences the events involoved in DNA transcription numbers above are ______, _______, ________, ______, and ________
88Sequential binding of RNA polymerase II-TFIIF complex, TFIIE, and TFIIH completes ___________________ formation. A.pre-initiation complexb.TF recognition elementc.pre-elongation complexD.TATA binding complex 89Which of the following is the GDP-GTP exchange protein?A.EF-Tub.none of the abovec.EF-TsD.EF-G 90RNA polymerase II has 14 subunits. Yesorno
Strand directed mismatch repair corrects copying erros based on Which of the following apply to the statement above, there maybe more than one. a. the strand containg breaks in the sugar backbone of the replicated DNA b. structurral instabilities created between oncorrect base pairing and associated histone proteins c. mutations that are evolutionary beneficial to an organims and recults in these areas of the genetic code to be turned off d. methylation of adenosine in GATC sequences during bacterial DNA replication e. repair sequence motifs that attract DNA polymerase gamma
Strand directed mismatch repair corrects copying erros based on which ones apply to sentence above? a. the strand containg breaks in the sugar backbone of the replicated DNA b. structurral instabilities created between oncorrect base pairing and associated histone proteins c. mutations that are evolutionary beneficial to an organims and recults in these areas of the genetic code to be turned off d. methylation of adenosine in GATC sequences during bacterial DNA replication e. repair sequence motifs that attract DNA polymerase gamma
A scientist compares the promoter regions of two genes. Gene A’s core promoter plus proximal promoter elements encompasses 70bp. Gene B’s core promoter plus proximal promoter elements encompasses 250bp. Which of the scientist’s hypotheses is most likely to be correct? More transcripts will be made from Gene B Transcription of Gene A involves fewer transcription factors Enhancers control Gene B’s transcription Transcription of Gene A is more controlled than transcription of Gene B.
Substrate Binding by RNA Polymerase RNA polymerase has t binding sites for ribonucleoside triphosphates: the initiation site and the elongation site. The initiation site has a greater Km for NTPs than the elongation site. Suggest what possible significance this fact might have for the control of transcription in cells.
A binding .site for RNA polymerase is called a ___ . a. gene b. promoter c. codon d. protein
Multiple Matching. Fill in the blanks with the words below that apply.18. ________ site of protein synthesis19. ________ carries the codon20. ________ carries the anticodon21. ________ a process synonymous with mRNA synthesis22. ________ bacteriophages participate in this transfer23. ________ duplication of the DNA molecule24. ________ process in which transcribed DNA code is deciphered into a polypeptide25. ________ involves plasmidsreplicationtRNAconjugationribosometransductionmRNAtranscriptiontransformationtranslationnone of these PreviousNext
91The mRNA Shine-Dalgarno sequence interacts with 26S rRNA anti-Shine-Dalgarno sequence. Yesorno 92Core polymerase (α2ββ’) use intact double-stranded DNA as a template. Yesorno 93Protein synthesis machinery reads the mRNA nucleotide sequence in groups of three bases or ______________. A.operatorsb.codonsc.open reading framesD.operons
Which statements are true? Explain why or why not.1 The consequences of errors in transcription areless severe than those of errors in DNA replication.2 Since introns are largely genetic “junk,” they do nothave to be removed precisely from the primary transcriptduring RNA splicing.3 Wobble pairing occurs between the first positionin the codon and the third position in the anticodon.4 During protein synthesis, the thermodynamics ofbase-pairing between tRNAs and mRNAs sets the upperlimit for the accuracy with which protein molecules aremade.5 Protein enzymes are thought to greatly outnum-ber ribozymes in modern cells because they can catalyzea much greater variety of reactions and all of them havefaster rates than any ribozyme.
The RNA polymerase from bacteriophage T7 diff ers structurally from prokaryotic and eukaryotic RNAPs and is extremely specifi c for its own promoter. Why do these properties make T7 RNAP useful in experiments with recombinant DNA?