Determine the amino acid sequence of a polypeptide from the following data:Complete hydrolysis of the peptide yields Arg, 2 Gly, Ile, 3 Leu, 2 Lys, 2 Met, 2 Phe, Pro, Ser, 2 Tyr, and Val.Treatment with Edman’s reagent releases PTH-Gly.Carboxypeptidase A releases Phe. Treatment with trypsin yields the following four peptides:1. Gly-Leu-Tyr-Phe-Lys                  3. Val-Ile-Arg2. Ser-Met-Gly-Leu-Tyr-Lys           4. Met-Leu-Pro-Phe

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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Determine the amino acid sequence of a polypeptide from the following data:
Complete hydrolysis of the peptide yields Arg, 2 Gly, Ile, 3 Leu, 2 Lys, 2 Met, 2 Phe, Pro, Ser, 2 Tyr, and Val.
Treatment with Edman’s reagent releases PTH-Gly.
Carboxypeptidase A releases Phe.

Treatment with trypsin yields the following four peptides:
1. Gly-Leu-Tyr-Phe-Lys                  3. Val-Ile-Arg
2. Ser-Met-Gly-Leu-Tyr-Lys           4. Met-Leu-Pro-Phe

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