Differentiate the following artificial media used in animal cell culture: serum-containing media serum-free media xeno-free media chemically-defined media protein-free media
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Differentiate the following artificial media used in animal cell culture:
serum-containing media
serum-free media
xeno-free media
chemically-defined media
protein-free media
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- For a laboratory exercise of antibiotic susceptibility test in a disk-diffusion method. What are the concentrations of antibiotic applied in the test?Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for? What tool is used to inoculate this medium?
- What is the reason for using sterilized filter paper in CHORIOALLANTOIC MEMBRANE (CAM) Assay?The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 6.8 x 106 cells/ml. Each well in a 6 well plate requires 2 x 105 cells. How should the solution be diluted so that 1ml can be added to each well?Discuss the role of the addition of ammonium chloride potassium lysius buffer in splenocyte culture preparatrion.
- Not only is blood agar an enriched medium that can support fastidious organism growth, but it can also serve as a differential medium in the identification of streptococcal species because of the presence of red blood cells that can be destroyed by an organism's hemolysins. Looking at this photo, how could you describe the organism growing on the surface of this blood agar plate? Multiple Choice a) Gamma-hemolytic b) Alpha-hemolytic c) Non-hemolytic d) Beta-hemolyticCentrifugation is the first step in most fractionations, but it separates only components that differ greatly in size. Compare and contrast very high, high, medium, and low-speed centrifugations of supernatant mixtures. Define the following: SDS Polyacrylamide-Gel Electrophoresis and ImmunoprecipitationIf you have to prepare 4 nM solution of human IgG ( immunoglobuline) which has molecular mass of 150 kDa. How many times do you need to dilute you stock solution of hIgG ( 1 mg/mL) please solve