Differentiate the Guanidium-thiocyanate phenol-chloroform method from the silica membrane based technology. What are the advantages or disadvantages of using commercially available kits compared to conventional RNA extraction techniques?

Q: Give advantages and disadvantages of using commercially available kits for plasmid extraction over…

A: As molecular biology techniques get more sensitive, a DNA extraction kit that yields high-quality…

Q: Explain the methodology, rationale, and challenges in the control of Triatomine vectors.

A: Triatomine: Triatominae bugs are the vectors of Chagas disease. These belong to the Reduviidae…

Q: Why safranin is used as the sample dye?

A: Safranin is utilized as a counterstain in some staining conventions, shading cell cores red. This is…

Q: Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays,…

A: Biuret, lowry, and bicinchoninic acid-based assays are used to quantify the protein concentration in…

Q: Alcohol is considered to be a significant reagent for the isolation of nucleicacids (RNA and DNA).…

A: In molecular biology, the extraction of nucleic acids  DNA and RNA is the most crucial method. DNA,…

Q: what is gel electrophoresis

A: Question : What is Gel electrophoresis,?

Q: Discuss about the reagents used for agarose gel electrophoresis

A: Gel electrophoresis is used to separate nucleic acid.

Q: What is the attached enzyme in this assay and its corresponding substrate? A. Horseradish peroxidase…

A: INTRODUCTION ELISA Enzyme linked Immunosorbent assay, is a technique used to detect antibodies in…

Q: What reaction would you expect when performing a positive control in the catalase assay? What would…

A: A catalase assay or test is implemented to differentiate streptococcus bacteria from staphylococcus…

Q: Provide the experimental procedure of peptide fragments by standard fluorenylmethoxycarbonyl (Fmoc)

A: The Fmoc method is a solid phase synthesis method for the generation of the different peptides…

Q: Why were radioactive sulfur and phosphorous used to label bacteriophage in Hershey and Chase's…

A: Alfred Hershey and Martha chase did so in order to label the protein coat of virus with radioactive…

Q: Can anyone assist in providing a detailed description of each step of the following purification…

A: Pectinase are group of enzymes which catalyzes the degradation of pectic substances. It occurs…

Q: in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this…

A: Answer: Introduction: Ribosomes are big complexes of RNA and protein which do the vital task of…

Q: At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How…

A: Plasmid training is a way of DNA extraction and purification for plasmid DNA. Many techniques were…

Q: Define about agarose gel electrophoresis ?

A: Electrophoresis is an important tool in biology. It involves separating basic biomolecules: nucleic…

Q: What are the importance of accurate wavelength measurement in purified DNA sample?

A: DNA is a double helical hereditary molecule. The nucleosome is a monomer of the DNA.  In DNA, four…

Q: theraphy, What are the drawbacks of this technology? Enumerate at least 2and briefly explain ypur…

A: Gene therapy can be defined as the introduction of a new DNA into an old DNA to bring about genetic…

Q: Can two-dimensional gel electrophoresis be used as a purificationtechnique? Explain.

A: Gel electrophoresis is a laboratory technique, which is used to separate mixtures of DNA or RNA…

Q: What other method could be used to purify lysozyme from white using its unusual pl?

A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…

Q: What do you mean by polyacrylamide gel electrophoresis (PAGE)  technique ?

A: To explain: To explain about polyacrylamide gel electrophoresis (PAGE)  technique and its uses

Q: What is the Availability of semi-synthetic/synthetic analogues regarding Callystatin A (a marine…

A: Introduction: callystatin A is a polyketide isolated from the sponge callyspongia truncata.

Q: What are the advantages and disadvantages of differential centrifugation?

A: differential as the name suggests is used to separate heterogenous solutions into their subsequent…

Q: What is the mechanism of separation of large DNA fragments in pulsed field gel electrophoresis.

A: Pulse Field Gel Electrophoresis is a powerful genotyping technique used for the separation of large…

Q: What is the principle of the gel electrophoresis separation technique?

A: Biological techniques are procedures or strategies for studying biological things. Biological…

Q: Why differential centrifugation can cause contamination in pellet?

A: Differential centrifugation is a typical process used in microbiology and cytology to separate…

Q: Give three reasons why liposomes/nanoparticles are attractive drug delivery system for recombinant…

A: Introduction: Liposomes are a small-sized vesicle sharing structural and compositional similarity…

Q: What is reductive dechlorination

A: Biodegradation refers to the transformation of complex organic compounds by bacteria, fungi, and…

Q: What are the different applications of Gel Electrophoresis?

A: The variation in size and charge of distinct molecules in a sample is exploited by the gel…

Q: Discuss the underlying biochemical principle of the nucleic acid sequencing methods known as…

A: One of the techniques for figuring out the sequence of DNA or RNA is called next-generation…

Q: Explain about dideoxy chaintermination sequencing ?

A: Dideoxy chain termination method is also called as Sanger sequencing. This method can be designed…

Q: what are two differences between agarose gel electrophoresis and SDS PAGE?

A: Agarose gel electrophoresis and the SDS page technique are used to separate the molecules. The…

Q: why does a higher agarose concentration render better resolution/separation of smaller DNA…

A: Electrophoresis is an essential lab procedure at the molecular level that uses an electric field to…

Q: Explain gel electrophoresis?

A: DNA is a prerequisite for all life. It contributes to heredity, protein-coding, and the genetic…

Q: What is the criticalphotoperiod? How can thecritical photoperiod relate toflowering be…

A: Plants are mainly multicellular organisms, predominantly photosynthetic eukaryotes of the kingdom…

Q: In the isolation of DNA which of the following best describe the purpose of using NaCl-SDS solution?

A: DNA extraction is a technique for separating DNA from cell membranes, proteins, and other cellular…

Q: What is reductive dechlorination and why is it environmentallyrelevant?

A: Chlorination refers to the process of the addition of chlorine into the water for the disinfecting…

Q: Process of Dpph free radical scavenging assay

A: DPPH free radical scavenging assay is a fast, basic, reasonable and broadly utilized technique to…

Q: Briefly describe the principle and the procedure of organic (Phenol-Chloroform) DNA extraction…

A: DNA Extraction: It is the process of isolating DNA from a cell. There are several techniques…

Q: .outline the processes used in the preparation of agarose gel of 1.5 concentration 5.outline the…

A: Disclaimer: Since you have asked multiple question, we will solve the first question for you. If you…

Q: how does polyacrylamide gel electrophoresis with SDS separate proteins ?

A: Answer: SDS = Sodium Dodecyl Sulfate is a detergent used in the polyacrylamide gel electrophoresis…

Q: What causes the separation of DNA fragments during agarose gel electrophoresis?

A: The technique of Gel electrophoresis is used or the separation of DNA fragments or macromolecules…

Q: Using the SDS-PAGE and the calibration curve given, calculate the size of the lysozyme and ovalbumin…

A: Rf is the retention factor that measures the distance traveled by a particular molecule. It allows…

Q: How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein  purification…

A: Protein purification is a process of isolation of specific protein from the complex protein mixture…

Q: Discuss the advantage and disadvantage of Wijs method of iodine value determination

A: Iodine value: The iodine value of an oil/fat is the number of grams of iodine absorbed by 100g of…