Differentiate the heat shock method from the electroporation process of transformation. Which method do you think will give a higher efficiency of transformation?

Q: (d) Bacteria strain that cannot grow on minimal medium but can grow on minimal medium with lactose…

A: Answer: MINIMAL MEDIA : It is the defined media that has composition of nutrients for microorganisms…

Q: Describe how an appropriate inoculum can be usedto successfully start an enrichment culture.

A: Enrichment culture is culturing a specific microorganism using certain growth media that favors…

Q: fluorogenic methods that can be used in detecting coliforms and E.coli

A:

Q: Why are biochemical tests used to identify microbes?

A: Microbes are living organisms that are very small and can be seen only with the help of a…

Q: Why is it necessary to perform an enrichment procedure in isolating Staphylococcus aureus instead of…

A: Staphylococcus aureus is a Gram-positive round-shaped bacterium that is normally found in the upper…

Q: What is the similarities of differential centrifugation and gradient centrifugation?

A: Similarities between differential centrifugation and gradient centrifugation is process involved…

Q: How can the enrichment culture technique be used medically?

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: Since the development of the calcium chloride transformation procedure, other procedures have been…

A: Bacterial transformation is the transfer of  DNA released from a donor bacterium into the…

Q: Describe the four steps that are usually carried out in micropropagation

A: BASIC INFORMATION    TISSUE CULTURE   In this a tissue is  made to grow in the laboratories that in…

Q: Which of the following is similar to a block of highly purified “jello-like” substance that is not…

A: Micropippete is laboratory equipment used to transfer small quantities of liquid. Restriction…

Q: Explain about different types of bioreactors and how they are used in cell suspension culture. What…

A: Ans: Bioreactor It refers to any device that supports the biological environment. This is used for…

Q: Compare and contrast precipitation reactions and agglutination reactions.

A: The immune system comprises cells, tissues, organs, molecules, and various processes, provides…

Q: micro-organisms are the most resistant to heat sterilization?

A: Most of the bacteria cannot survive adverse heat condition. But somehow particular bacteria can…

Q: Toreterate, in your own words what is 1. The purpose of the selective factor in combination media? 2…

A: In microbiology, one of the most important components is the medium. Microorganisms such as…

Q: Describe in short the process of size exclusion chromatography (molecular exclusion chromatography)

A: Size exclusion chromatography is a separation of biomolecules techniques where separation was done…

Q: How would you prepare 10 to the power -4 dilution from a culture?

A: Bacterial culture or generally microbial culture is a method of multiplying the microorganism by…

Q: What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for…

A: Enrichment culture media is the one which is used for the growth and isolation of a specific type of…

Q: Using a schematic diagram, summarize the following steps in preparing competent cells for…

A: prepare competent cells for transformation ;- Add 20 µL of each ligation reaction or 1 ng of uncut…

Q: Staphylococcus aureus in Foods Why is it necessary to perform an enrichment procedure in isolating…

A: Staphylococcus aureus is a member of the firmicutes, a Gram-positive round-shaped bacterium, which…

Q: If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: If a component is heat sensitive, how might you conveniently achieve sterility of that medium? a.…

A: Option A i.e. filtration

Q: What are the possible ways to sterilize heat labile products? Explain your answer with suitable…

A: Heat labile materials are those substances that can undergo change rapid change when exposed to high…

Q: In theory, if a 1-liter culture of rich medium is inoculated with 3 bacterial cells per milliliter,…

A: Generation time is the time taken for the doubling of the single cell. According to data given in…

Q: What is meant by the term “enrichment culture”?

A: The culture medium or growth medium is the specific medium, which contains all essential nutrients,…

Q: What is meant by recalcitrance in microbiology? Give an example

A: Now a days environmental pollution is one of the major issue  and it  is defined as any undesirable…

Q: Distinguish batch culture and continuous culture

A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…

Q: For each mode of bioreactor operation listed below, name one advantage and one disadvantage. a.…

A: The three different types of bioreactors have their own set of advantages and disadvantages, one…

Q: With the aid of a diagram, explain the principles of gel filtration chromatography.

A: Gel filtration chromatography: Chromatography is an analytical technique where the components of a…

Q: What are the main types of immobilization techniques used in biosensors? (with a deep explanation)

A: Immobilization It is a technique that is used for the chemical and physical fixation of the…

Q: What reaction would you expect when performing a positive control in the oxidase assay? What would…

A: Oxidase assay is performed to test the presence of the cytochrome c oxidase enzyme in bacteria. A…

Q: You treat cells briefly with radioactively labeled cysteine and then transfer them to a medium with…

A: Cysteine is a nonessential amino acid. It is one of the amino acid that contains sulfur which allows…

Q: Discuss the functions of the following components in performing gel electrophoresis;…

A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…

Q: What is the result of this biochemical test in the gelatin deep?  Which enzyme is this testing for?…

A: Gelatin Hydrolysis Test: Gelatin is a collagen-derived protein obtained from the connective tissues…

Q: Why is egg enrichment not added to BPA prior to sterilization?

A: Baird–parker agar or BPA is a selective medium is identify or isolate S. aureus in food. It contains…

Q: Give two reasons for why heat fixing is used?  Adherence of bacterial cells to the slide…

A: Haet fixing is the procedure in gram staining of the bacteria. This has to be done so that the…

Q: What is micropropogation? Mention its advantages.

A: With the advancement in science, scientists are creating new methods to create plants artificially…

Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: Which of the following items is the least suitable for sterilization by autoclave? a. Water for…

A: An autoclave is a machine that is used to perform industrial and scientific processes that require…

Q: Write the limitations of sterilization by Dry heat?

A: The dry heat sterilization technique includes the removal or killing the microorganism and require…

Q: What would happen if the enrichment method in the isolation of bacteriophage was omitted?the…

A: The bacteriophages are bacteria that are infected by a virus are known to exist in all possible…

Q: escribe the examples of biochemical test that are used to identify the bacteria in the laboratory.

A: According to the definition of a biochemical test, it is a test that is used to distinguish between…

Q: What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can…

A: Aseptic preparation of culture media :-

Q: Suppose your Professor handed you a test tube with 2.0mL of E.coli broth culture and told you to…

A: In both basic and applied research, "microbiology" is a highly significant area. Proteomics,…