What reaction would you expect when performing a positive control in the oxidase assay? What would it mean if a known oxidase-positive bacterium did not cause the expected reaction?
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- What reaction would you expect when performing a positive control in the oxidase assay? What would it mean if a known oxidase-positive bacterium did not cause the expected reaction?
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- Biochemical tests such as the fermentation test can be used when unknown bacterial cells are identified. true or false?Give 2 fluorogenic methods that can be used in detecting coliforms and E.coli.What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for? What tool is used to inoculate this medium?
- What is the result of this biochemical test in the gelatin deep? Which enzyme is this testing for? What tool is used to inoculate this medium? Gelatin positive liquid, use needle, protease,Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours. Which of the following can be interpreted from this result? A. The organism did not grow so lysine decarboxylase activity could not be determined. B. The organism is negative for lysine decarboxylase. C. The media is too acidic to determine lysine decarboxylase activity. D. The organism is positive for lysine decarboxylaseFor an experiment where you will using different concentrations of avocado catalase to determine how that affects the rate of the reaction with its substrate, hydrogen peroxide, what would be the general protocol? What is the control in the experiment?
- In the Ames test shown in Figure 16-17, what is the reason for adding the liver extract to each sample?If you mix two unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is used.Briefly explain how you could use the color of the Coomassie assay sample solution to determine if anunknown sample needs to be diluted in order to measure the sample’s concentration
- Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?A mistake was made in preparing the arginine decarboxylase test, and mineral oil was NOT added to the test tube after inoculating with bacteria. If an arginine decarboxylase-positive bacteria species was inoculated into the above arginine-decarboxylase broth without mineral oil, what would likely be the color result of this bacteria in this test? Would this produce a false positive or false negative result? Explain.What medium is this? What is the result in tube at left? In second tube? What does it tell you about the biochemical abilities of these organisms? What is the reagent used to detect the red?