Since the development of the calcium chloride transformation procedure, other procedures have been developed. One is called electroporation. Using your textbook or other sources, describe this procedure for transformation
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- In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?Briefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatographyWhich of the following techniques takes advantage of NET CHARGE to purify/characterize proteins? a. Isoelectric focusing b. Hydrophobic chromatography c. Affinity chromatography d. DialysisExplain.
- What is the basis for the separation of proteins by the following techniques? (a) gel-filtration chromatography (b) affinity chromatography (c) ion-exchange chromatography (d) reverse phase HPLCWhat is the driving force behind paper chromatography?Which of the following scientists use this X-ray crystallography?
- If you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.In which type of chromatography, solvents of increasing polarity are passed through a column of silica gel?a) High performance liquid chromatography) Thin layer chromatography) Adsorption chromatography) Gas-liquid chromatographyWhat is the basic purpose of the paper chromatography?
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