direction of water flow across the membrane
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- If you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?Where would the following substances partition in water containing palmitic acid micelles? (a) +H3N—CH2—COO−, (b) +H3N—(CH2)11—COO−.0.0 M sucrose = _______ 0.008_____________ g/min 0.4 M sucrose = _______ -0.013_____________ g/min 0.6 M sucrose = ________ -0.023g____________ g/min 0.8 M sucrose = ________-0.026____________ g/min 1.0 M sucrose = ________ -0.033____________ g/min Can i please have the step by step calculations Using the osmosis data in the question above, determine the percent weight change for each sucrose solution . Write your answer in standard notation and use 1 digit past the decimal point - e.g. 8.6. (Note that the last zero in the answer is considered to be a digit) 0.0 M sucrose = _________ ___________% 0.4 M sucrose = _______ ______________% 0.6 M sucrose = _____________________% 0.8 M sucrose = __________________% 1.0 M sucrose = ____________________%
- In a 0.1000 M acetic acid solution at 25 degrees celsius , the acid ionizes to the extent of about 1.34 %. Since each molecule of acetic acid which ionizes produces 1 H+ ion and 1 C2H3O2- ion, the concentration in the solution are: HC2H3O2 < -----------> H+ + C2H3O2-We have isolated a mixture of 2 protein of the same molecular weight but different isoelectric points. The isoelectric points are 7.5 for Protein A and 9.0 for Protein B. What would be the order of elution from a cation exchange column if a buffer of pH 7 is used? Group of answer choices Both proteins will remain in the column. Both proteins will elute simultaneously Protein B followed by Protein A Protein A followed by Protein BHow many grams of glucose (C6H2O6 molecular mass =180daltons) would be present in one liter of a 1M (molar) solution of glucose?
- Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.If the osmotic pressure of a 7.96×10-2-M aqueous solution of Fe2(SO4)3 was found to be 8.71 atm at 20°C, what would be the "observed" van't Hoff factor? Use the above van't Hoff factor to predict the freezing point of this solution.Note that the corresponding molality would be 8.25×10-2 m, given that the density of the solution is 0.997 g/cm3. °C Use the above van't Hoff factor to predict by how many degrees the boiling point of this solution will be elevated above that for pure water.Suppose the major solutes in intact lysosomes are KCl (~0.1 M) and NaCl (~0.03 M). When isolating lysosomes, what concentration of sucrose is required in the extracting solution at room temperature (25oC) to prevent swelling and lysis? Calculate how much sucrose (342.3 g/mol) would you need to make 500 ml of this extracting solution.
- The greatest buffering capacity at physiological pH would be provided by a protein rich in which of the following amino acids? Choose one from among the possible answers and explain. Serine Cysteine Alanine HistidineStudent A says that he can insert any value of optical density in the equation of standard curve to determine the corresponding value of protein content. Does the student say it right or wrong? Explain why?Solution A: 200 mM glucose; solution B: (100 mM NaCl + 50 mM KCl). Which of these two solutionswill have the highest osmotic pressure and why?