DNA MELTING Two antiparallel single strands form a DNA duplex according to the following model: SA SB SASH a) You have carried out melting studies at various concentrations for an unknown DNA. Here is the fitted data (1) (ii) (iii) (iv) " y=0.0000271x + 0.00282. Based on the best-fit equation above, find the unknown sequence from the options below given the free energy data at 37°C. Show your work AGGCGATCGG and its complement AGCAATGGCC and its complement GGAGAACTTA and its complement GGCCAAGGCC and its complement AºG - 9.50 kcal/mol 35.97 kJ/mol 30.55 kJ/mol -10.20 kcal/mol - b) Thermodynamically speaking, AS and AH can both drive processes. What happens to these parameters as double stranded DNA is melted? Explain.

DNA MELTING Two antiparallel single strands form a DNA duplex according to the following model: SA SB SASH a) You have carried out melting studies at various concentrations for an unknown DNA. Here is the fitted data (1) (ii) (iii) (iv) " y=0.0000271x + 0.00282. Based on the best-fit equation above, find the unknown sequence from the options below given the free energy data at 37°C. Show your work AGGCGATCGG and its complement AGCAATGGCC and its complement GGAGAACTTA and its complement GGCCAAGGCC and its complement AºG - 9.50 kcal/mol 35.97 kJ/mol 30.55 kJ/mol -10.20 kcal/mol - b) Thermodynamically speaking, AS and AH can both drive processes. What happens to these parameters as double stranded DNA is melted? Explain.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 1P: Semiconservative or Conservative DNA Replication If 15N-Iabeled E. coli DNA has a density of 1.724...

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