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does melting point determination remove impurites in crude protein?
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- Please provide the solution to the attached imageA supporting electrolyte is usually added to the analyte solution to minimize solution resistance.minimize solution conductivity.make the analyte less soluble in solution.increase the scan rate.Arrange the following steps in the correct order. I. Prepare the separatory funnel. II. Mix and release pressure. III. Add the solution to be extracted and add the extracting solvent. IV. Collect the organic layer. a. I, II, III, and IV b. I, III, IV, II c. I, III, II, IV d. IV, II, I, III
- Pick all that are TRUE regarding analysis of quaternary structures of proteins using polyacrylamide electrophoresis:I. The added β-mercaptoethanol disrupts S--S bonds bridging the polypeptide chains causing the appearance of higher Rf bands compared to the native protein run. II. Heating up any protein before subjecting to SDS-PAGE will always result in the formation of more than one band.III. A good asymmetrical gel layout would be : (Lane 1) MW ladder, (2) native protein, (3) protein + β-ME, (4) protein + HCL, (5) protein + β-ME + HCl.IV. Formation of a single band in the protein + β-ME + HCl run, whose Rf is lower than the native run, could be indicative that the protein is a homodimer.A. I onlyB. I and IIC. II and IIVD. None is true.pls explainUse special gel loading tips or a micro-syringe to load the complete sample in a narrow well. Take care not to poke the well bottom with the tip as this will create a distorted band. Never overfill wells. This could lead to poor data if samples spill into adjacent wells, and poorly resolved bands. Load 20-40 μg total protein per mini-gel well. -If you have a lysate with protein concentration of 1500 ug/mL, how much sample you need to load according to the step indicated in D.3Calculate the bulk density of a 110 cm3 soil sample that has a 25 percent water content (gravimetric or mass basis) and weighs 150 g.
- You finish doing an experiemnt with Benzoin. These are the results you get:Boiling start point: 137 ceciusBoiling end point: 130 ceciusVile while empty (with cap on): 15.348Vile with crude prod (with cap on: 15.748 Your starting weight: 1.00115.748 - 15.348 = 0.400 / 1.001 = 0.3996 or 39.96% YieldAnswer the following questions with this information:Initial Mass of impure sample:Mass of recrystallized benzoin:Percent reccovery of Benzoin:Melting pot range of purified benzoin:Literature melting point for benzoin:Show complete solution pls.Mention the relative sizes of hard gelatin capsule with their corresponding volume and fill weight at 0.6g/cm3 powder density