During cheese production, LAB convert lactose to lactate and casein (milk protein) to amino acids. Lactate and amino acids then become the substrates for further microbial growth, which results in aroma production and deacidification of the cheese. The yeast Yarrowia lipolytica grows on the surface of many cheeses; it is capable of both lactate and amino acid catabolism. When grown on a lactate plus amino acid medium, Y. lipolytica preferentially consumes amino acids. Amino acid degradation results in the release of ammonia, which increases the pH. Draw a flow chart that shows the LAB fermentation of milk, followed by the growth of Y. lipolytica. Indicate which substrates are consumed first and what happens to the pH. Based on this simplified scenario, why do you think most cheeses involve the activity of more than one yeast species?

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.5: Blood Glucose And Hemoglobin A1c
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During cheese production, LAB convert lactose to lactate and casein (milk protein) to amino acids. Lactate and amino acids then become the substrates for further microbial growth, which results in aroma production and deacidification of the cheese. The yeast Yarrowia lipolytica grows on the surface of many cheeses; it is capable of both lactate and amino acid catabolism. When grown on a lactate plus amino acid medium, Y. lipolytica preferentially consumes amino acids. Amino acid degradation results in the release of ammonia, which increases the pH. Draw a flow chart that shows the LAB fermentation of milk, followed by the growth of Y. lipolytica. Indicate which substrates are consumed first and what happens to the pH. Based on this simplified scenario, why do you think most cheeses involve the activity of more than one yeast species?

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