You were asked to perform an experiment using spectrophotometric analysis. The system that you are studying is a reaction catalyzed by the enzyme TDET and requires the coenzyme GWRM. In the reaction, substance SHARE is converted to a colored substance CRMSN that can be detected spectrophotometrically. All other substances will not absorb at the Amax of CRMSN. Test tube set-ups were prepared as follows: T1: SHARE + TDET T2: SHARE + TDET + GWRM T3: SHARE + TDET + GWRM + gradual heating until boiling T4: SHARE + TDET + GWRM + WARN* T5: TDET + GWRM *Substance WARN can bind to GWRM and prevent it from binding to TDET. QUESTION: Will product CRMSN form over time in the tubes you prepared? Complete the table below: Write a brief explanation Do you expect product CRMSN to be formed over for your answer. time? [Yes/No] T1 T2 T3 T4 T5

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter6: Biomedical Electrodes, Sensors, And Transducers
Section: Chapter Questions
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helping tags: Biochemistry, spectrophotometric analysis, enzyme, substrate, coenzyme

Will upvote, just pls help me answer the table. Thanks.

You were asked to perform an experiment using spectrophotometric analysis. The
system that you are studying is a reaction catalyzed by the enzyme TDET and requires
the coenzyme GWRM. In the reaction, substance SHARE is converted to a colored
substance CRMSN that can be detected spectrophotometrically. All other substances
will not absorb at the Amax of CRMSN.
Test tube set-ups were prepared as follows:
T1: SHARE + TDET
T2: SHARE + TDET + GWRM
T3: SHARE + TDET + GWRM + gradual heating until boiling
T4: SHARE + TDET + GWRM + WARN*
T5: TDET + GWRM
*Substance WARN can bind to GWRM and prevent it from binding to TDET.
QUESTION: Will product CRMSN form over time in the tubes you prepared?
Complete the table below:
Write a brief explanation
Do you expect product
CRMSN to be formed over for your answer.
time? [Yes/No]
T1
T2
T3
T4
T5
Transcribed Image Text:You were asked to perform an experiment using spectrophotometric analysis. The system that you are studying is a reaction catalyzed by the enzyme TDET and requires the coenzyme GWRM. In the reaction, substance SHARE is converted to a colored substance CRMSN that can be detected spectrophotometrically. All other substances will not absorb at the Amax of CRMSN. Test tube set-ups were prepared as follows: T1: SHARE + TDET T2: SHARE + TDET + GWRM T3: SHARE + TDET + GWRM + gradual heating until boiling T4: SHARE + TDET + GWRM + WARN* T5: TDET + GWRM *Substance WARN can bind to GWRM and prevent it from binding to TDET. QUESTION: Will product CRMSN form over time in the tubes you prepared? Complete the table below: Write a brief explanation Do you expect product CRMSN to be formed over for your answer. time? [Yes/No] T1 T2 T3 T4 T5
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