L-lysine is being produced by Corynebacterium glutamicumin a bioreactorusing glucose as the carbon source, ammonia as the nitrogen source. The bacteria grow aerobicallyin a continuous process at steady- state.All streams of this process are aqueous. In the inlet stream, 8 grams of glucose is included per 100 grams of stream liquid, which flows at a rate of 500 kg/h. The flow rate of glucose in the outlet steam is 1 kg/h. The reaction occurs at 25 °C. (a)What is the mole percentageof glucose that was consumed during cell growth inside the bioreactor? (b)Does the growth of Corynebacterium glutamicum cells inside this bioreactor release or absorb heat?Should heat be supplied to the bioreactor, or removed from, to maintain the operationtemperature?

L-lysine is being produced by Corynebacterium glutamicumin a bioreactorusing glucose as the carbon source, ammonia as the nitrogen source. The bacteria grow aerobicallyin a continuous process at steady- state.All streams of this process are aqueous. In the inlet stream, 8 grams of glucose is included per 100 grams of stream liquid, which flows at a rate of 500 kg/h. The flow rate of glucose in the outlet steam is 1 kg/h. The reaction occurs at 25 °C. (a)What is the mole percentageof glucose that was consumed during cell growth inside the bioreactor? (b)Does the growth of Corynebacterium glutamicum cells inside this bioreactor release or absorb heat?Should heat be supplied to the bioreactor, or removed from, to maintain the operationtemperature?

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

See similar textbooks

Similar questions

L-lysine is being produced by Corynebacterium glutamicum in a bioreactor using glucose as thecarbon source, ammonia as the nitrogen source. The bacteria grow aerobically in a continuous process atsteady-state. All streams of this process are aqueous. In the inlet stream, 8 grams of glucose is included per100 grams of stream liquid, which flows at a rate of 500 kg/h. The flow rate of glucose in the outlet steamis 1 kg/h. The reaction occurs at 25 °C. What is the mole percentage of glucose that was consumed during cell growth inside thebioreactor?
L-lysine is being produced by Corynebacterium glutamicumin a bioreactorusing glucose as the carbon source, ammonia as the nitrogen source. The bacteria grow aerobicallyin a continuous process at steady-state.All streams of this process are aqueous. In the inlet stream, 8 grams of glucose is included per 100 grams of stream liquid, which flows at a rate of 500 kg/h. The flow rate of glucose in the outlet steam is 1 kg/h.The reaction occurs at 25 °C. According this does the growth of Corynebacterium glutamicum cells inside this bioreactor release or absorb heat? Should heat be supplied to the bioreactor, or removed from, to maintain the operation temperature?
L-lysine is being produced by Corynebacterium glutamicum in a bioreactor using glucose as the carbon source, ammonia as the nitrogen source. The bacteria grow aerobically in a continuous process at steady-state. All streams of this process are aqueous. In the inlet stream, 8 grams of glucose is included per 100 grams of stream liquid, which flows at a rate of 500 kg/h. The flow rate of glucose in the outlet steam is 1 kg/h. The reaction occurs at 25 °C. (a) What is the mole percentage of glucose that was consumed during cell growth inside the bioreactor? (b) Does the growth of Corynebacterium glutamicum cells inside this bioreactor release or absorb heat? Should heat be supplied to the bioreactor, or removed from, to maintain the operation temperature?
L-lysine is being produced by Corynebacterium glutamicum in a bioreactor using glucose as the carbon source, ammonia as the nitrogen source. The bacteria grow aerobically in a continuous process at steady-state. All streams of this process are aqueous. In the inlet stream, 8 grams of glucose is included per 100 grams of stream liquid, which flows at a rate of 500 kg/h. The flow rate of glucose in the outlet steam is 1 kg/h. The reaction occurs at 25 °C.What is the mole percentage of glucose that was consumed during cell growth inside the bioreactor?Does the growth of Corynebacterium glutamicum cells inside this bioreactor release or absorbheat? Should heat be supplied to the bioreactor, or removed from, to maintain the operation temperature?
Can xylane powder from beechwood be dissolved in M9 media? I'm evaluating the production of hydrogen with E. coli (it has a construction that alows this strain to depolimerize xylane into xylose monomers by an endoxylanase), but i have a limited amount of this xylane, and i read TB, AM1 & M9 mediums were used, but they don't say if they just poured the xylan powder into the media solution, i also read they autoclaved it with a soft acid solution and then neutralized it with a basic soluction, but this is an acid hydrolyzate (AH) plus enzymatic hydrolysis (EH). It ´s certain that using both would enhance available xylose concentration, but i want to evaluate EH by itself, and i still can't find if it is just added as powder to the media. Would it dissolve? do i have to add some kind of buffer to the media?.
A genetically modified bacterium was cultivated to produce 1,3-propanediol (1,3-PDO), and the following yields were obtained: YX/S = 0.106 gX/gS and YP/S = 0.28 gP/gS. Knowing that the culture medium was formulated with glucose, ammonia and some mineral salts, and the cultivation was carried out under aerobic conditions, answer:a) What is the oxygen demand in this process?b) What is the maximum theoretical yield of 1,3-PDO in this process?c) Projecting the scale-up, what cell concentration must be reached to obtain 10 kg of 1,3-PDO in a batch reactor with 1 m3 of culture?
TOPIC: Production of Glutamic acid Pathway -Fermentative production of poly (γ-glutamic acid) from renewable carbon source and downstream purification through a continuous membrane-integrated hybrid process. Conditions: Batch, Aerobic Microorganisms: Corynebacterium glutamicum (NCIM 2168) Inoculum: 10%v/v NCIM-2168 Chemical equation: 2 C6H12O6 + 2 NH3 + O2→ C5H9NO4 + C5H7NO2+ 2CO2+ 7H2O Question: Please explain clearly on the upstream and downstream process that involve in this pathway.
The protein catalase is an enzyme that catalyzes the decomposition of hydrogen peroxide:2 H2O2 (aq) → 2 H2O (l) + O2 (g)and has a Michaelis-Menten constant of 25 × 10-3 mol·dm-3 and a turnover number of 4.0×107s-1.The total enzyme concentration is 0.016×10-6 mol·dm-3 and the initial substrate concentration is4.32×10-6 mol·dm-3 Calculate the maximum reaction rate (????) for this enzyme, and the initial rateof this reaction. Note that catalase has a single active site.
You have decided to design a CSTR with recycle to degrade TCE (trichloroethylene) cometabolically by aerobic phenol-utilizing bacteria. To do this, you will feed phenol (C6H5OH; as a C source) and oxygen, along with required nutrients, to the reactor in order to grow a population of bacteria that can fortuitously degrade TCE. The problem is to first determine what mass of bacteria is required in the reactor to effect the TCE destruction desired, and then to determine how much phenol and oxygen must be fed to the reactor to maintain that population. The waste characteristics are as follows: Q0 = 5 m3/d, C0 = 100 μg/L TCE (influent concentration), X0 = 0 mg/L. The Xa selected for design is 1500 mg VSSa/L, and θx is 8 d. Kinetic coefficients for TCE decomposition are as follows: qTCE = 0.015 mg TCE/mg VSSa, KTCE = 0.5 mg/L. Kinetic coefficients for phenol degraders are Y = 0.8 g VSSa/g phenol, b = 0.1/d, q = 1 g phenol/g VSSa•d, and K(phenol) = 1 mg/L. 2.1. Determine the reactor size…
During cheese production, LAB convert lactose to lactate and casein (milk protein) to amino acids. Lactate and amino acids then become the substrates for further microbial growth, which results in aroma production and deacidification of the cheese. The yeast Yarrowia lipolytica grows on the surface of many cheeses; it is capable of both lactate and amino acid catabolism. When grown on a lactate plus amino acid medium, Y. lipolytica preferentially consumes amino acids. Amino acid degradation results in the release of ammonia, which increases the pH. Draw a flow chart that shows the LAB fermentation of milk, followed by the growth of Y. lipolytica. Indicate which substrates are consumed first and what happens to the pH. Based on this simplified scenario, why do you think most cheeses involve the activity of more than one yeast species?
Aerobic degradation of an organic compound by mixed cultureof organism in wastewater can be represented by following reaction. C3H6O3 + a O2 + b NH3 → c C5H7NO2 + d H2o + e CO2 A. Determine a, b, c, d and e, if YX/S = 0.4 d X/g S. B. Determine the yield coefficients YX/O2 and YX/NH3. C. Determine the degree of reductions for the substrate, bacteria and RQ for the organisms
Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.