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- TLC spots are colored, they can be observed by however, if they colorless they can be seen saing.Project 2: Food Dye Spectroscopy How can you use serial dilutions to determine the relationship between concentration and absorbance? Graphs may also help in this explanation.Project 2: Food Dye Spectroscopy What is the relationship between absorbance and transmittance?
- Carotenoids are yellow, orange, andred pigments synthesized by plants. Theobserved color of an object is not thecolor of light it absorbs but rather thecomplementary color, as described by acolor wheel such as the one shown here.On this wheel, complementary colorsare across from each other. (a) Based onthis wheel, what color is absorbed moststrongly if a plant is orange? (b) If a particular carotenoid absorbsphotons at 455 nm, what is the energy of the photon?Eq 6 to solve is in the other picture, it's not 2 different questions just in case it gets flagged for being in two separate pictures.Project 2: Food Dye Spectroscopy What method will you use to assess the absorbance-concentration relationship? (The most obvious method is to see if there is a linear relationship between the absorbance and the concentration. What methods do you know that might reveal such a linear relationship?)