Given the following values, use the absorbance formula to find the hemoglobin concentration of the unknown. As =0.600 Au =0.300 Cs =20g/dl 2. Convert 12.8 g Hb into percent Hb 3. Compute the grams Hb of 88% Hb.
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1. Given the following values, use the absorbance formula to find the
hemoglobin concentration of the unknown.
As =0.600
Au =0.300
Cs =20g/dl
2. Convert 12.8 g Hb into percent Hb
3. Compute the grams Hb of 88% Hb.
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- Suppose you start with a 175 mL solution. After adding 2 mL of titrant, you spectrophotometer returns a reading of 0.500 au. What is the true absorbance after correcting for the dilution effect?A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound?A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound? I need help with part B of this question.
- The glucose content of samples can be determined using Nelson’s test. The equation of the line for the standard curve prepared for varying concentrations of glucose is y = 3.0x + 0.2. Based on this information, determine the glucose concentration (in M) of a mango preserve having an absorbance reading of 0.891.Sample (μg) Absorbances (520 nm) Concentration 0.0 0.621 0 2.5 0.599 2.5 5.0 0.556 5 10.0 0.469 10 15.0 0.418 15 20.0 0.291 20 Plot a graph in which y axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors.A 0.1 g amine-containing compound is dissolved in water then diluted to 100 mL. You subject it to spectroscopic analysis, in order to get the concentration of amine in this compound. Next, you get 1 mL of the previously diluted sample then dilute it again to 250 mL for measurement. Then, you fill 3/4 of a 1-cm cuvette with this diluted sample, and you run an analysis using an AAS. The recorded absorbance is 0.545 at 410 nm. What is the molecular weight of the compound? (The molar absorptivity is 1.23 x 104 cm-1 mol-1 L.)
- How do I find Concentration? flask Calculate solution Concentration (M) Volume stock of 0.40 M CuSO4 (ml) Volume of flask (ml) Absorbance at 635 nm(a.u.) 1 2.0 10 .185 2 4.0 10 .362 3 6.0 10 .510 4 8.0 10 .674 5 10.0 10 .843Part C: Copper. A 0.275 M solution of Cu^2+ gives an absorbance reading of 0.788 at 620 nm. Another solution gives an absorbance reading of 0.340 under the same conditions. What is the concentration of the second solution in moles of Cu^2+/L and grams of Cu^2+/LSolution. Vol. of A solution (mL) [methyl red], M Absorbance 1 10.00 _1.64 x10^-16_ _0.256_ 2 15.00 _2.45 x10^-16_. _0.373_ 3 20.00 _3.27 x10^-16_. _0.486_ 4 25.00 _4.09 x10^-16_. _0.620_ Plot a graph of the absorbance (y axis) as a function of the concentration of methyl red (x axis). What is the slope of the graph?
- Determine the concentration of your prepared Iron (III) Salicylate Dianion solution (pure ASA) in the 10.0 mL of 1.0 M NaOH in mg/mL (Part D) using Beer's Law plot. Hint:You need to use the measured absorbance reading of your prepared Iron (III) dianion (absorbance reading: 0.83). Information from the plot: Standard: Concentration ofASA in Standards (mg/mL) A: 0.18 B: 0.14 C: 0.10 D: 0.070 E: 0.040 Mass of salicylic acid (g) 0.17g Volume of acetic anhydride (mL) 0.34mL Mass of filter paper (g) 0.35gMass of "impure" ASA and filter paper (g) 0.49g Mass of prepared "impure" ASA (g) 0.14g Melting point range of prepared ASA (°C) 20.9 °C Melting point range of standardASA (°C) 18.5°C Percent purity: 28.57% Percentage yield: 66.7% Chemical equation: C7H6O3 + C4H6O3pure ASA knowing that two consecutive dilutions were done to prepare the solution. Show all of your work.C9H8O4 + C2H4O2 b) Then, calculate the mass (in mg) ofAn unknown sample of Cu2+ gave an absorbance of 0.475 in an atomic absorption analysis. Then 5.00 mL of solution containing 75.0 ppm (= µg/mL) Cu2+ was mixed with 45.0 mL of unknown, and the mixture was diluted to 500.0 mL in a volumetric flask. a) Write an expression for the final concentration after dilution. b) Calculate the final concentration of the added standard. c) The absorbance of the new solution was 0.987. Find the initial concentration of Cu2+ in the unknown sampleThe nickel level in a soil sample was determined using AAS. A 3.0 g sample was dissolved in acid and then diluted to a total volume of 100.0 mL. The absorbance of this solution determined at a wavelength of 232 nm, was found to be0.3. Several standard solutions were tested under the same conditions and a calibration curve shown below was obtained. Calculate the mass of nickel in mg.
