Explain the expressions of primary culture, cell haH, and continuous cell haH. Explain the reason why some cells grow in suspension in culture and some grow by adhering. Give examples of these two cell types.

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Plant tissue is a cluster of related cells that work together to fulfil a specific purpose for the…

Q: Describe the events that occur with E. coli in each of the following growth conditions: in a medium…

A: E. coli is a bacteria that has about three thousand genes. The bacteria E. coli has a set of three…

Q: b. The growth rate of a certain species of bacteria is being modelled to determine the number of…

A: The bacteria and archaea reproduce by binary fission, multiple fissions spore production. It takes…

Q: List 5 physical methods of controlling microbial growth, and give an example of each. Why is moist…

A: Heat is one of the best and commonly used physical methods to control microbial growth. By heating…

Q: DRAW IT Draw the following growth curves for E. coli, starting with 100 cells with a generation time…

A: Here, the growth curve will be drawn by plotting Time on X-axis and growth on Y-axis.

Q: describe the steps involved in primary cell dissociation protocol, assessing cell viability and sub…

A: Answer :- As we know that Cell viability examines give a readout of cell wellbeing through…

Q: In the laboratory, autoclaving is required to prepare media for the growth of What type of method of…

A: The most effective method by which we sterilize the laboratory instruments is known as autoclaving.

Q: Write instruction to make cell culture medium using DMEM consist of 10% FBS, 1% streptomycin.

A: Introduction Streptomycin is an antimycobacterial drug which is used in the treatment of…

Q: The principle of Grams staining based on the cell wall composition of bacterial cell. Justify the…

A: Gram stain divides the bacteria into two major categories Gram positive bacteria and Gram Negative…

Q: You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells…

A: We shall be performing a dilution calculation for this question. We know, C1V1 = C2V2 C1 is the…

Q: Explain the mechanism of action of several of the physical and chemical agents employed to control…

A: Microbes such as bacteria, viruses, worms may act as nuisance causing various diseases in plants,…

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…

A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…

Q: Differentiate between a microbistatic and microbicidal agent.

A: Microbicidal and microbiostatic generally fall within the antibacterial scope. The toxic gas…

Q: Explain about different types of bioreactors and how they are used in cell suspension culture. What…

A: Ans: Bioreactor It refers to any device that supports the biological environment. This is used for…

Q: The cell concentration measured in samples taken at certain intervals from a microorganism growth…

A: From the given table,   Cf, final cell concentration =0.376  Ci, initial cell concentration=0.01…

Q: Why after a certain period the growth rate of the culture decreases until growth stops?

A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: What are types of cell culture media?

A: A cell culture medium is solid or liquid in nature. It is a mixture of all necessary nutrients…

Q: n this step, wash the cells with ice-cold PBS. Drain the PBS, then add ice-cold lysis buffer.…

A: The question you have asked lacks some background information about what you are trying to do, But…

Q: The factors for the manipulation of culture medium?

A: Culture medium It is also called a growth medium. It is a solid, liquid, or semi-solid medium that…

Q: A growth medium consisting of only known amounts of water, magnesium, calcium chloride, potassium…

A: The growth medium, also called as Nutrient Broth, is a kind of solution which is sterilized to get a…

Q: Why does a one-step growth curve differ in shapefrom that of a bacterial growth curve?

A: Growth curves are generally the description of the density of cell populations in liquid culture…

Q: Why does turbidity in a cultured media losses its reliability at high cell concentrations?

A: turbidity is used as a measure of cell density within a culture sample.

Q: Provide comparison and illustration of expected growth curves from a pure culture with the…

A: 1. The growth curve from a pure culture represents the number of live cells in a bacterial…

Q: What is the effect of trypsin EDTA solution on monolayer cell culture preparation?

A: Trypsin is an enzyme which helps in digestion of proteins.In the small intestine,proteins are broken…

Q: Draw and label the set-up of Manual paraffin wax infiltration

A: Digram of set-up of Manual paraffin wax infiltration. As per our guidelines we are not allowed to…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Cell organelles extraction  The process to obtain cell organelles from the cell.

Q: The graph shows the number of microbial cells (log scale) against time in a liquid culture. In which…

A: Bacterial growth is divided into four stages. During the lag phase, the bacterium prepares itself by…

Q: 1a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and…

A: Every cell culture has a unique or specific pH at which the cells in the culture will grow. PH is an…

Q: Describe each phase of the BACTERIAL GROWTH CURVE (explain what happens, give the cells…

A: Bacteria grow rapidly under a favorable environment where all the necessary conditions for its…

Q: Of what practical importance are air borne microorganisms to the laboratory workers?  What…

A: Questions related to the microbiology labs.  Note: each questions are answered under particular…

Q: Shown below is the growth of E. coli in nutrient agar plates after exposure to different doses of UV…

A: Bacterial growth Growth is defined as an increase in cell number/ amount on the culture plate. To…

Q: b. The growth rate of a certain species of bacteria is being modelled to determine the number of…

A: Controlled laboratory studies on the growth of microorganisms have resulted in invaluable…

Q: What is a complex medium? and What are the basic components of a culture medium?

A: Medium is defined as the solid or liquid substratum on which the cells or organ can grow for further…

Q: Define the following termonologies in brief :  Focal Adhesion Biofilm  Calcification and examples…

A: "Since you have asked multiple parts of the same question, we are eligible to answer only the first…

Q: Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go…

A: The bacteria are classified according to their cell wall composition into Gram positive and Gram…

Q: Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking.…

A: Streaking method This method is used to produce isolated colonies of bacteria. This helps in…

Q: When bacteria are grown under adverse conditions, i.e., in the presence of a poison such as an…

A: Introduction Bacteria are single-celled organisms with a small size. Bacteria can be found…

Q: Which has a greater impact on the survival of an organism, temperatures above its maximum…

A: The microbes grow in a wide temperature range. The microbial growths are found to be higher at their…

Q: In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar…

A: The disc diffusion method was developed by Kirby and Bauer to determine the efficiency of a…

Q: Give two reasons for why heat fixing is used?  Adherence of bacterial cells to the slide…

A: Haet fixing is the procedure in gram staining of the bacteria. This has to be done so that the…

Q: compare the cell sizes of the untreated (1st photo) and colchicine-treated (2nd photo) Allium cepa.

A: Colchicine is an alkaloid produced by plants like Gloriosa superba of Lilliaceae family.It…

Q: Define the meaning of Suspension cell culture

A: Cell culture is a media which is used for growth of the cells.

Q: main goal of motility agar

A: MICROBIAL GROWTH MEDIA- It is the culture media that serves as a nutrient media for the growth of a…

Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…

A: Culture consists of appropriate substances which provides the growth to the desired organisms.

Q: If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a…

A: Serial dilution  It is a method of stepwise dilution of a concentrated solution to get the desired…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."