discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.
discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.
Want to see the full answer?
Check out a sample Q&A here
Related Biology Q&A
Find answers to questions asked by students like you.
Q: Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing…
A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: Can you please discuss the principles of using Motility Test Medium for the detection of bacterial…
A: Bacterial motility is usually demonstrated in a semi-solid agar medium. Motile bacteria ‘swarm' in…
Q: Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different…
A: The liquid or solid substratum on which, the organ or cell explants can be grown. The medium…
Q: For Staphylococcus aureus, indicate which tests need to be done & what media you need. Fill in box…
A: Staphylococcus aureus is a spherical-shaped cocci that belongs to the family of Firmicutes. It is…
Q: Which one of the following methods of antimicrobial susceptibility testing combines some of the…
A: Antimicrobial susceptibility tests are performed to identify which drugs are effective against a…
Q: Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.
A: Methyl Red (MR) and Voges-Proskauer (VP) broth is used as the medium in which both the Methyl Red…
Q: Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.
A: Click to see the answer
Q: WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE…
A: Indole test is a biochemical test for distinguishing between Enterobacter aerogenes and Escherichia…
Q: Kligler’s iron agar and SIM are multiple test media.. What component of both media allows you to…
A: Kligler’s Iron Agar (KIA) test is used in differentiating certain members of the Enterobacteriaceae…
Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…
A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…
Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…
A: The bacteria that belong to the genus Enterobacter are opportunistic pathogens. Enterobacter…
Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…
A: Coliform bacteria are the gram negative, facultative anerobic, and non-spore-forming rod. They can…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear
A: Click to see the answer
Q: You were given a mixed nutrient agar broth culture of bacteria 1a. How will you determine the…
A: A mixed bacterial culture consists of more than one bacterial cell types growing together. On the…
Q: Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features…
A: Staphylococcus aureus and Streptococcus pyogenes are gram positive bacteria that cause infections in…
Q: You are testing for Escherichia coli in the sample. What media would you use? and Describe typical…
A: A medium in microbiology is defined as a solid or liquid substance used to culture the…
Q: Clear tubes in the MIC test were inoculated in Mueller-Hinton Agar to determine the MBC of the…
A: MBC - stands for minimum bactericidal concentration It is the lowest concentration of antibiotics…
Q: Which isolation technique is best used in isolating microorganisms (in countable good plates) from a…
A: The phrase "isolation" in microbiology refers to the separation of a strain from a natural, mixed…
Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…
A: A growth medium is a also known as a culture medium which can be solid, liquid, and even…
Q: What test can help you differentiate staphylococci from streptococci? What reagent or media would be…
A: Both are gram positive bacteria.
Q: When the new batch of culture media is prepared, why it is recommended to test the AST using the…
A: Introduction Culture is referred to the process of growing the cell outside the animal or plant…
Q: Provide three reasons why the use of aseptic technique is essential when handling microbial cultures…
A: Aseptic technique: This technique is used to prevent contamination like bacteria, viruses, fungi,…
Q: Provide three reasons why the use of aseptic technique is essential when handling microbial cultures…
A: Aseptic techniques means using practices and procedures to prevent contamination from pathogens. It…
Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…
A: The practices and procedures that are used to prevent contamination from pathogens are referred to…
Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: name the genus and species of 2 enteric bacteria that do not ferment lactose discuss why a mixed…
A: Hi! As you have posted multiple questions and not mentioned which one of them to be answered, I will…
Q: Which one of the following methods of antimicrobial susceptibility testing requires a dilution…
A: Antimicrobial susceptibility testing is a method which is used for determination of antimicrobials…
Q: If a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium…
A: Manitol Salt Agar (MSA) in used in lab, mostly for selective and differential growth of microbial…
Q: How is each of the following media used for the detection of coliforms?a. lactose broth with Durham…
A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…
Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?
A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…
Q: Agar was not completely set prior to inoculating with the organism. Effect: Rationale:
A: Bacterial cultivation requires specific types of medium that can provide some of the basic…
Q: Which one of the following methods of antimicrobial susceptibility testing combines some of the…
A: Answer: Antimicrobial Susceptibility test is the method of determining the susceptibility of a…
Q: Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least…
A: Introduction :- Micro-organisms are classified into different categories on the basis of the…
Q: What concentration of agar is used in a semisolid medium for motility determination? How does that…
A: Agar is a polysaccharide that is obtained from marine seaweeds. Agar is mostly used in food and…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: how to find out the test is differential or selective in the microbiology lab test?
A: Many kinds of medias are used in microbial study. Two types of media having similar inferred names…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…
A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…
Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…
A: Motility is the ability of the organism to move. Eukaryotic organisms use the cilia, pseudopodia,…
Q: Name one other medical diagnostic are being used in reagent test strips
A: Reagent test strip are diagnostic strips that contain specific reagents for various organ diagnosis…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Why is Muller Hinton Agar (MHA) ideal for antibiotic susceptibility testing
A: MHA(Mueller Hinton Agar) was developed by Mueller and Hinton in 1941. It is used to test antibiotic…
Q: Make a viable count of the donor culture by spot inoculation of he nutrient agar plate WITHOUT…
A: Nutrient agar is generally preapared for the bacterial growth to carry out biochemical reations on…
Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?
A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…
Q: Describe several moist heat methods and their chief applicationsfor sterilization and disinfection.
A: A temperature higher than the normal temperature or suitable temperature for the growth of the…
Q: The medium shown here was inoculated with a bacterium then incubated for 48 hours; an uninoculated…
A: A control tube is used to minimize the variable factors other than the one being tested.
Q: how many CFUs would be presented in 10 mL of the original culture
A: In microbiology, colony-forming unit or CFU refers to a unit to estimate the number of living or…
Q: Which of the following is used in the sterilization of the medium? a) benmarge b) swab c) autoclave…
A: Sterilization is the process of making something (instrument, swab, room etc. ) free of microbes…
Q: Which of the following is true Select one: O a. Inoculating loop and needle are transfer equipment…
A: Culture medium is the primary production environment for biological products. The pilot or scale-up…
Q: discuss the clinical significance, principles, reagents (if any) and positive and negative results…
A: *Note: Since you have posted a question with multiple sub-parts, we will solve first three sub-parts…
